Techniques to improve detection and analysis of extracellular vesicles using flow cytometry

HC Inglis, A Danesh, A Shah, J Lacroix… - Cytometry Part …, 2015 - Wiley Online Library
Extracellular vesicles (EVs) range in size from 50 nm to 1 µm. Flow cytometry (FCM) is the
most commonly used method for analyzing EVs; however, accurate characterization of EVs …

Understanding extracellular vesicle diversity–current status

DW Greening, RJ Simpson - Expert review of proteomics, 2018 - Taylor & Francis
ABSTRACT Introduction: Extracellular vesicles (EVs) represent an important mode of
intercellular communication. There is now a growing awareness that predominant EV …

Revealing the diversity of extracellular vesicles using high-dimensional flow cytometry analyses

G Marcoux, AC Duchez, N Cloutier, P Provost… - Scientific reports, 2016 - nature.com
Extracellular vesicles (EV) are small membrane vesicles produced by cells upon activation
and apoptosis. EVs are heterogeneous according to their origin, mode of release …

Extracellular‐vesicle isolation from different biological fluids by size‐exclusion chromatography

M Monguió‐Tortajada, M Morón‐Font… - Current Protocols in …, 2019 - Wiley Online Library
This unit describes how to isolate extracellular vesicles (EVs) from different biological fluids
using size‐exclusion chromatography (SEC) and how to prepare your starting sample and …

Flow cytometry analysis of circulating extracellular vesicle subtypes from fresh peripheral blood samples

M Marchisio, P Simeone, G Bologna, E Ercolino… - International journal of …, 2020 - mdpi.com
Extracellular vesicles (EVs) are released by shedding during different physiological
processes and are increasingly thought to be new potential biomarkers. However, the …

[HTML][HTML] Diagnostic and prognostic potential of extracellular vesicles in peripheral blood

ALS Revenfeld, R Bæk, MH Nielsen, A Stensballe… - Clinical …, 2014 - Elsevier
Abstract Purpose Extracellular vesicles (EVs) are small, membrane-enclosed entities
released from cells in many different biological systems. These vesicles play an important …

Mass-spectrometry-based molecular characterization of extracellular vesicles: lipidomics and proteomics

S Kreimer, AM Belov, I Ghiran, SK Murthy… - Journal of proteome …, 2015 - ACS Publications
This review discusses extracellular vesicles (EVs), which are submicron-scale, anuclear,
phospholipid bilayer membrane enclosed vesicles that contain lipids, metabolites, proteins …

Methods for the physical characterization and quantification of extracellular vesicles in biological samples

DLM Rupert, V Claudio, C Lässer, M Bally - Biochimica et Biophysica Acta …, 2017 - Elsevier
Background Our body fluids contain a multitude of cell-derived vesicles, secreted by most
cell types, commonly referred to as extracellular vesicles. They have attracted considerable …

Extracellular vesicle isolation methods: rising impact of size-exclusion chromatography

M Monguió-Tortajada, C Gálvez-Montón… - Cellular and Molecular …, 2019 - Springer
Extracellular vesicles (EVs) include a variety of nanosized vesicles released to the
extracellular microenvironment by the vast majority of cells transferring bioactive lipids …

Molecular evaluation of five different isolation methods for extracellular vesicles reveals different clinical applicability and subcellular origin

RE Veerman, L Teeuwen, P Czarnewski… - Journal of …, 2021 - Wiley Online Library
Extracellular vesicles (EVs) are increasingly tested as therapeutic vehicles and biomarkers,
but still EV subtypes are not fully characterised. To isolate EVs with few co‐isolated entities …