Recombinant DNA technology, which has provided techniques for the routine isolation and
analysis of genes from almost any organism, was originally successfully employed during …

Many filamentous fungi of all taxa can now be subject to DNA-mediated transformation.
Many dominant selectable markers are available and the range available is increasing as …

The protoplast method for the transformation of filamentous fungi is described in detail, as is
the Restriction Enzyme-Mediated Integration (REMI) procedure for introducing tagged …

In comparison with transformation vectors available for use in E. coli or yeast, there has
been relatively little development of vectors for use in filamentous fungi. For example …

Filamentous fungi include a large, heterogeneous group of heterotrophic organisms with
profound influence in human activities. In spite of their economic and scientific relevance …

Molecular genetic manipulation of micro‐organisms requires the development of plasmid‐
mediated transformation systems that include:(i) infusion of exogenous DNA into recipient …

The advent of techniques for manipulating and isolating genes from plant pathogenic fungi
has lead to a rapid increase in our understanding of how these organisms cause disease I1l …

Filamentous fungi have been used for decades in the commercial production of enzymes,
antibiotics, and specialty chemicals. Traditionally, improving the yields of these products has …

A major prerequisite for molecular genetic research in filamentous fungi is the availability of
a gene transfer system. This consists of two main components: a vector containing a …

Recombinant DNA technology offers one the potential to make well-defined alterations in
the genetic make-up of an organism. The development of this technology was based on two …