The ability to accurately quantify specific nucleic acid molecules in complex biomolecule
solutions in real time is important in diagnostic and basic research. Here we describe a DNA …

We report the design, synthesis, and characterization of binary oligonucleotide probes for
mRNA detection. The probes were designed to avoid common problems found in standard …

We report that TO-PRO-3, a thiazole orange analogue with a trimethine bridge, functions as
a deep-red fluorescent indicator for the internal loop structure of the bacterial (Escherichia …

We report here a new small molecule fluorogen and RNA aptamer pair for RNA labeling.
The small-molecule fluorogen is designed on the basis of fluorescently quenched …

Ribosome assembly has been studied intensively using Förster resonance energy transfer
(FRET) with fluorophore-labeled fragments of RNA produced by chemical synthesis …

Interrogating non-coding RNA structures and functions with small molecules is an area of
rapidly increasing interest among biochemists and chemical biologists. However, many …

The forced-intercalation peptide nucleic acid (FIT-PNA) concept, introduced by Seitz and co-
workers, is based on replacing a nucleobase of the PNA sequence with a cyanine dye (such …

Efficient detection and observation of dynamic RNA changes remain a tremendous
challenge. However, the continuous development of fluorescence applications in recent …

Fluorogenic RNAs such as the Mango aptamers are uniquely powerful tools for imaging
RNA. A central challenge has been to develop brighter, more specific, and higher affinity …

Efficient detection and observation of dynamic RNA changes remain a tremendous
challenge. However, the continuous development of fluorescence applications in recent …