To determine whether a specific nucleotide sequence is required to direct polyadenylation of
a simian virus 40 early pre-mRNA in a soluble HeLa whole-cell lysate, we constructed a …

Abstract mRNA-specific polyadenylation can be assayed in vitro by using synthetic RNAs
that end at or near the natural cleavage site. This reaction requires the highly conserved …

Conditions have been developed which allow for the efficient, accurate, and specific
polyadenylation of exogenously added mRNA precursors in a wholecell lysate derived from …

The sequence AAUAAA is found near the polyadenylation site of eucaryotic mRNAs. This
sequence is required for accurate and efficient cleavage and polyadenylation of pre-mRNAs …

To study the mechanism and factors required to form the 3'ends of polyadenylated mRNAs,
we have fractionated HeLa cell nuclear extracts carrying out the normally coupled cleavage …

Exogenous RNA containing the simian virus 40 early polyadenylation site was efficiently
and accurately polyadenylated in in vitro nuclear extracts. Correct cleavage required ATP. In …

We have partially purified a poly (A) polymerase (PAP) from HeLa cell nuclear extract which
is involved in the 3′-end formation of polyadenylated mRNA. PAP had a molecular weight …

It is well known that nearly all eukaryotic mRNAs contain a 3′ poly (A) tail. A
polyadenylation signal (AAUAAA) nearby the 3′ end of pre-mRNA is required for poly (A) …

The hexanucleotide AAUAAA has been demonstrated to be part of the signal for cleavage
and polyadenylation at appropriate sites on eucaryotic mRNA precursors. Since this …

Three sequences in the vicinity of poly (A) addition sites are conserved among vertebrate
mRNAs. We analyze the effects of single base changes in each position of AAUAAA and in …