[HTML][HTML] Live-seq enables temporal transcriptomic recording of single cells

W Chen, O Guillaume-Gentil, PY Rainer, CG Gäbelein… - Nature, 2022 - nature.com
Live-seq can address a broad range of biological questions by transforming scRNA-seq
from an end-point to a temporal analysis … the transcriptome of a live cell. Before exploring this …

Monitoring the spatio-temporal organization and dynamics of the genome

HA Shaban, A Seeber - Nucleic acids research, 2020 - academic.oup.com
… Another example from live cell imaging of DNA DSB dynamics in budding yeast showed
that the spatial chromatin organization differ at various time scales (11,12). Therefore, it is …

A perspective view on the nanomotion detection of living organisms and its features

L Venturelli, AC Kohler, P Stupar… - Journal of Molecular …, 2020 - Wiley Online Library
… These nanometric scale oscillations can be detected by depositing living cells onto a
micro-fabricated cantilever and by monitoring its displacements with an atomic force microscope-…

DNA-based nanostructures for live-cell analysis

SB Ebrahimi, D Samanta, CA Mirkin - Journal of the American …, 2020 - ACS Publications
… The earliest analysis techniques based on DNA, … of cells, respectively, preventing their use
for live-cell analysis. The introduction of linear DNA probes that can study events in live cells, …

Temporal integration of mitogen history in mother cells controls proliferation of daughter cells

M Min, Y Rong, C Tian, SL Spencer - Science, 2020 - science.org
… In this work, we combined live-cell imaging with temporally controlled perturbations to … cells.
Contrary to the textbook model that cells sense mitogen availability only in the G 1 cell cycle …

Automated segmentation and tracking of mitochondria in live-cell time-lapse images

AEYT Lefebvre, D Ma, K Kessenbrock, DA Lawson… - Nature …, 2021 - nature.com
… We first assign each mitochondrion in the first temporal frame to a new track. In each
subsequent temporal frame, we assign each mitochondrion to either an existing track or to a new …

Advanced near‐infrared light for monitoring and modulating the spatiotemporal dynamics of cell functions in living systems

G Chen, Y Cao, Y Tang, X Yang, Y Liu… - Advanced …, 2020 - Wiley Online Library
… mice, the living cells could be distinguished from dead cells in situ, because the living cells
have both … The spatial and temporal modulation of cells has generated great interest among …

[HTML][HTML] Spatiotemporal coordination of transcription preinitiation complex assembly in live cells

VQ Nguyen, A Ranjan, S Liu, X Tang, YH Ling… - Molecular cell, 2021 - cell.com
… Here we use live-cell, single-molecule tracking in S. cerevisiae to visualize constrained …
Our study provides a global spatiotemporal model for transcription initiation in live cells. …

Transcription in living cells: molecular mechanisms of bursting

J Rodriguez, DR Larson - Annual review of biochemistry, 2020 - annualreviews.org
… It is possible to observe nascent transcription in live cells using the RNA stem-loop and …
Nearly all our knowledge of transcriptional bursting in living cells comes from the MS2 imaging …

Dynamic real-time imaging of living cell traction force by piezo-phototronic light nano-antenna array

Q Zheng, M Peng, Z Liu, S Li, R Han, H Ouyang… - Science …, 2021 - science.org
… the force distribution across a single living cell remains a challenge. … temporal resolution of
333 ms have been demonstrated for force mapping. The dynamic mapping of cell force of live