… mRNAs of catabolic genes expressed from the TOL plasmid are spatially organized. … putida
mt-2 stemming from the TOL operons of plasmid pWW0, we adopted an RNA-FISH approach …

… mt-2 RB1401 and KG1206 contain the intact TOL plasmid and P u -lux and P m -lux fused
plasmid… and lower operon of the pWW0 (TOL plasmid), respectively. These are responsible for …

… strain containing either the pOK12 vector (p) or ptolA plasmid, the Δpal strain containing
either the pOK12 vector (p) or the ppal plasmid were spotted on LB agar with or without 1 mM Cr(…

… pSW-2 was taken as a negative control for plasmid stability in P. putida. This experiment
was performed with three biological replicates, and error bars represent standard deviation. …

… putida mt-2, which utilizes toluene … TOL pathway, which are highly likely localized on the
chromosome of the strain under study, have been so far described only within the TOL plasmids. …

… the soil bacterium Pseudomonas putida mt-2, in which the key … into a set of standard plasmid
vectors, the inducibility of the … ·pTn7-R (Table 2), and plasmid pTn7-M was amplified with …

… mt-2 carries the catabolic pWW0 plasmid, which endows cells with the ability of processing
toluene, xylenes, and ethylbenzene via the TOL … plasmid-borne xyl genes of the lower TOL …

… This is of interest considering our previous proposal that the Tol-Pal system helps … absence
of FtsN, we examined the localization of plasmid-encoded TolQ-GFP in cells of strain CH238 (…

… Both the Tol ORF and the C57BL/6 Tol strain used in this work are available upon … For
secondary vaccinations, mice received a single DNA tattoo with 20 μl of the 2 μg/μl plasmid …

… Therefore, we believe that these four RM systems limit the electrotransformation of Tol 5
with plasmids propagated in E. coli. To validate this hypothesis, electroporation was conducted …