… reflect the assembly of structurally distinct ubiquitin chains on … to the active site cysteine in
an ubiquitin-conjugating enzyme (E2), … Substrate selection depends on ubiquitin ligases (E3). …

… of a superfamily of cullin‐dependent ubiquitin ligases, which … for recruiting the ubiquitin‐conjugating
enzyme, whereas Skp1 … to express, purify, and assemble two well‐studied SCF …

… E1 ubiquitin-activating enzyme and E2 ubiquitin-conjugating … Cul2 is a member of the Cullin
protein family, which includes … do not assemble into the VHL complex and do not detectably …

… The cullin‐RING family of ubiquitin ligases regulates diverse … enzymes: E1 (ubiquitin activating),
E2 (ubiquitin conjugating) … ) according to the manufacturers' instructions. Analysis of the …

… members of RING components of Cullin-RING ligases (CRLs)… E1 ubiquitin-activating enzyme,
an E2 ubiquitin-conjugating … -F box protein) E3, to assemble the ubiquitin chain via the K48 …

… E1, and one of the two ubiquitin-conjugating E2 enzymes (… coli lysate according to the
instructions of the supplier (New … Cdc53/cullin and the essential Hrt1 RING-H2 subunit of SCF …

… Ubiquitin is then transferred to a ubiquitin conjugating … of cullins is that individual cullin
proteins may assemble various … vitro for p53 ubiquitination after incubating with El and E2-Ubc5. …

… of activated ubiquitin to E2 ubiquitin conjugating enzyme, … Assembly of an ubiquitin chain
on a substrate targets it to the … , as well as its assembly into active CDK2 complexes because …

… -conjugating enzyme Ubc5. In SCF … by VHL and SCF ubiquitin ligases is determined not
solely by the Cullin/Rbx1 … of target proteins by an E2 ubiquitin-conjugating enzyme. Elongin B, a …

… E3 ligase, the prototypical cullin family E3 ubiquitin ligase… activated ubiquitin is then transferred
onto an E2 ubiquitin conjugating … i2 0 CANDl, preventing SC FR OC i com plex assembly. …