… protein cross-linker explored in this study was based on a reaction recently discovered in our
group, namely the azide… occurs, along with reduction of the azide without cleavage.33 We …

… -cleavable cross-linker for the characterization of a multisubunit protein … aptly positioned
azido group in the spacer of a protein cross-linker for facile mapping of lysines in close proximity…

… acid pairs that are positioned in close proximity to each other. … rest of the cross-linker or
may contain another protein, if the … the length of the spacer in the cross-linker and the length of …

… Also, hydrophobic protein regions lacking lysines are not … in the peptide map after reduction
of the cross-linker. This … reducing agent: (i) The azide in the spacer of BAMG is reduced to an …

… , and MS-cleavable cross-linker with multistage tandem mass … mapping PPIs and their
interaction interfaces in living cells is critical not only for a comprehensive understanding of protein …

… of the protein or protein complex can be placed within the … From the length of the spacer of
the cross-linker, the … of the cross-linker used in our experiments enabled mapping of several …

… , the cross-linker may require additional functionality in its spacer … are highly reactive with
lysines and the N-termini of proteins, with … model that placed NusA into the unexplained density. …

… method not only for mapping protein-protein interactions of all … Our cross-linker targets lysine
residues and the free protein N… expanded azide-DSG (7.7 Å spacer length) and two lysine …

… a detailed linkage map, when isolated proteins are analyzed, … the cross-linker has reacted
with residues on both its ends, … bond joining the lysine ε-amine with the cross-linker or at the …

… change, we added the cross-linker directly in the culture … in which the azido group in the
spacer of the cross-linker has … score also placed δ in this region, but the remaining eight placed it …