… lamellae enables structural and ultrastructural studies of the cell interior in a near-native
state… These studies were enabled by the development and incremental improvement of lamella …

… capturing the structures without any targeting inside the lamellae is high. However, for smaller
… transformed to the coordinate frame of reference of the focused ion beam to guide milling. …

… the complex cellular environment using focused ion beam (FIB) … created a new field of structural
cell biology studies in which … model of the lamella that accounts for the lamella pretilt and …

… of features of interest, focused ion beam milling to make the … prevent ion beam erosion at the
front face of the lamella and … and the interaction with the ion beam transforms the condensed …

… thick for cryo-electron microscopy (cryo-EM). This allows visualizing cellular ultrastructures in
… limit for imaging biological samples, if cryo-ET is required, lamellae may be prepared with a …

… phase plate and cryo-focused ion beam milling have dramatically … In these cameras, a
scintillator layer converts electrons into … Thin lamellas are then prepared at the cryo-FIB (ion beam: …

… Focused-ion beam (FIB)-milling has become the method of … Positioned at the diffraction
plane, a phase plate converts the … lamella, using the same imaging conditions as above. While …

… ) localization of the targeted structures for cryo-ET data acquisition on the lamellas in the
cryo-… the biological features of interest (Figure 7A) were then predicted using the transformation …

… specimen shapes such as lamellae or needles that can be … for exploring 3D protein
structures at near-atomic resolution to … and running an affine transform to correct for minor …

… fundamental processes in molecular and structural biology. … markers onto the thin lamellae,
under cryogenic conditions, … The subtomograms are then transformed according to the …