Development of an asymmetric PCR-ELISA typing method for Citrus tristeza virus based on the coat protein gene
… The coat protein gene of isolates of citrus tristeza virus (CTV) from 20 citrus-producing
regions … An asymmetric PCR-ELISA typing (APET) assay was developed in the frame of this …
regions … An asymmetric PCR-ELISA typing (APET) assay was developed in the frame of this …
[PDF][PDF] Citrus tristeza virus (CTV) diagnosis and strain typing by PCR-based methods
G Nolasco - Options Méditerranéennes: Série B. Etudes et …, 2001 - om.ciheam.org
… a multiplicity of strains may develop. Diagnosis of CTV which … amounts of a cloned coat
protein gene of CTV isolate B53. … 0.075 for Taqman and asymmetric PCR ELISA respectively. …
protein gene of CTV isolate B53. … 0.075 for Taqman and asymmetric PCR ELISA respectively. …
Development and Application of a Multiplex Reverse-Transcription Polymerase Chain Reaction Assay for Screening a Global Collection of Citrus tristeza virus …
A Roy, G Ananthakrishnan, JS Hartung… - …, 2010 - Am Phytopath Society
… Development of an asymmetric PCR-ELISA typing method for Citrus tristeza virus based on
the coat protein gene… Methods 155:97-108. 33. Permar, TA, Garnsey, SM, Gumpf, DJ, and Lee…
the coat protein gene… Methods 155:97-108. 33. Permar, TA, Garnsey, SM, Gumpf, DJ, and Lee…
Asymmetric PCR ELISA: increased sensitivity and reduced costs for the detection of plant viruses
G Nolasco, Z Sequeira, C Soares, A Mansinho… - European Journal of …, 2002 - Springer
… of Citrus tristeza virus and Rupestris stem pitting-associated … Two strategies have been
developed for the rapid detection … The capsid protein gene of CTV was amplified from …
developed for the rapid detection … The capsid protein gene of CTV was amplified from …
Prevalence of severe Citrus tristeza virus infection in Khasi mandarin throughout the north-eastern states of India: an evidence study based on coat protein gene …
… base … gene sequence based phylogenetic analysis grouped present isolates into four clades
with reference severe isolates (ie VT, B165, HA and T3), indicating their probable evolution. …
with reference severe isolates (ie VT, B165, HA and T3), indicating their probable evolution. …
Epidemiological situation of Citrus tristeza virus in mainland Portugal
G Silva, G Nolasco - Phytopathologia Mediterranea, 2013 - JSTOR
… The asymmetric PCRELISA typing method based on the coat … ) developed an asymmetric
PCR-ELISA typing method based … Diversity of the coat protein gene of Citrus tristeza virus (CTV…
PCR-ELISA typing method based … Diversity of the coat protein gene of Citrus tristeza virus (CTV…
[PDF][PDF] Overview of Strain Characterization in Relation to Serological and Molecular Detection of Citrus tristeza Closterovirus
… PCR-ELISA being a laborious and expensive method was modified and simplified by using
… of citrus tristeza virus isolates from cyprus on the basis of the coat protein gene. Journal of …
… of citrus tristeza virus isolates from cyprus on the basis of the coat protein gene. Journal of …
[PDF][PDF] virus-indexing of citrus tristeza virus for screening quality planting material in Citrus spp.
DN Muske, SJ Gahukar, AA Akhare - Trends in Biosciences, 2015 - researchgate.net
… There are no molecular methods allowing reliable typing of CTV … (MMM) method was
developed for the PCR-based differentiation … in their coat protein genes. Phythopathology.86: 101. …
developed for the PCR-based differentiation … in their coat protein genes. Phythopathology.86: 101. …
Indexing Virus and Virus-Like Diseases of Citrus
… of the most devastating citrus viruses ie citrus tristeza virus. … IC-RT-PCR was used to
amplify the coat protein gene [112]. … PCR-ELISA being a laborious and expensive method was …
amplify the coat protein gene [112]. … PCR-ELISA being a laborious and expensive method was …
East Adriatic—a reservoir region of severe Citrus tristeza virus strains
… affecting the development of modern citrus production … may also be correlated with the type
of citrus growing practice. In … the analysis of Citrus tristeza virus coat protein gene sequences. …
of citrus growing practice. In … the analysis of Citrus tristeza virus coat protein gene sequences. …