[HTML][HTML] Encapsulation mechanisms and structural studies of GRM2 bacterial microcompartment particles

G Kalnins, EE Cesle, J Jansons, J Liepins… - Nature …, 2020 - nature.com
… system for recombinant GRM2 shell particles from Klebsiella … cryo-EM structure of pT = 4
BMC particle, demonstrate the … particular core enzymes in the core encapsulation process. …

Variety of size and form of GRM2 bacterial microcompartment particles

EE Cesle, A Filimonenko, K Tars, G Kalnins - Protein Science, 2021 - Wiley Online Library
… and its interactions with microcompartment shell. Exact mechanisms of BMC assembly are
… pneumoniae GRM2-like particles encapsulating signature enzyme choline lyase (CutC) and …

Modulation of hybrid GRM2-type bacterial microcompartment shells through BMC-H shell protein fusion and incorporation of non-native BMC-T shell proteins

EE Česle, K Ta̅rs, J Jansons… - ACS Synthetic …, 2023 - ACS Publications
… The microcompartment shell contains an encapsulated … In this work, we explore two BMC
shell modulation mechanisms: first, … Second, we produced microcompartment particles using …

Biochemical characterisation of the Glycyl Radical Enzyme Containing Microcompartment (GRM2) from Proteus mirabilis

G Moody - 2022 - discovery.ucl.ac.uk
… Bottlenecks impeding these efforts have been inefficient assembly and encapsulation of …
, and may have novel and different assembly mechanisms. As such, studying the GRM2 may …

Biophysical approaches to understand and re-purpose bacterial microcompartments

G Kumar, S Sinha - Current Opinion in Microbiology, 2021 - Elsevier
… than that of encapsulated enzyme and encapsulation provided … BMCs assemble via a
hierarchical mechanism, the size of the … They allow single particle tracking of protein molecule and …

Bacterial microcompartments

CA Kerfeld, C Aussignargues, J Zarzycki… - Nature Reviews …, 2018 - nature.com
… , the encapsulated proteins are structurally and functionally … : carbon dioxide-concentrating
mechanism protein CcmM and … the observed carboxysome-like particles were shown to have …

Advances in the world of bacterial microcompartments

AM Stewart, KL Stewart, TO Yeates, TA Bobik - Trends in biochemical …, 2021 - cell.com
… Recent structural studies of MCP proteins and recombinant … and mechanisms and raised
new questions about MCP structure. … encapsulated within a protein shell. MCPs are found in 23 …

Heterologous Assembly of Pleomorphic Bacterial Microcompartment Shell Architectures Spanning the Nano‐to Microscale

BH Ferlez, H Kirst, BJ Greber, E Nogales… - Advanced …, 2023 - Wiley Online Library
… standing of the molecular mechanisms governing their self-… a broad distribution of particle
sizes ranging from ~30 – 200 … , with one or more smaller shells encapsulated within a larger …

[HTML][HTML] Recent structural insights into bacterial microcompartment shells

JM Ochoa, TO Yeates - Current opinion in microbiology, 2021 - Elsevier
… nm in diameter, MCPs encapsulate and optimize a myriad of … Additional structural studies
on more remote BMC homologs … formation of larger particles, though their structures could not …

[HTML][HTML] Protein stoichiometry, structural plasticity and regulation of bacterial microcompartments

LN Liu, M Yang, Y Sun, J Yang - Current Opinion in Microbiology, 2021 - Elsevier
… proteinaceous shell and encapsulated enzymes that are … -associated microcompartments
(GRM), choline utilization (GRM2), … the underlying molecular mechanisms, these assembly and …