Termination of non‐coding transcription in yeast relies on both an RNA Pol II CTD interaction domain and a CTD‐mimicking region in Sen1

Z Han, O Jasnovidova, N Haidara, A Tudek… - The EMBO …, 2020 - embopress.org
… In addition, we show that Sen1-dependent termination strictly requires CTD recognition by …
action in the context of a kinetic competition between termination and elongation. We also find …

[HTML][HTML] Single-molecule characterization of extrinsic transcription termination by Sen1 helicase

S Wang, Z Han, D Libri, O Porrua, TR Strick - Nature Communications, 2019 - nature.com
… statistical and kinetic analysis of Pol II interactions with a … the kinetics of extrinsic eukaryotic
transcription termination … cerevisiae RNA Polymerase II (Pol II) was purified from a strain that …

[PDF][PDF] Sen1 is a key regulator of transcription-driven conflicts

U Aiello, D Challal, G Wentzinger, A Lengronne… - Molecular Cell, 2022 - cell.com
RNA polymerase II at TRCs within genes and the rDNA and at sites of transcription-transcription
We therefore first analyzed Sen1-dependent alterations on RNAPII distribution focusing …

[HTML][HTML] Genome-wide mapping of yeast RNA polymerase II termination

P Schaughency, J Merran, JL Corden - PLoS genetics, 2014 - journals.plos.org
Pol II maintaining a pool of free enzyme able to compete for unoccupied promoters. Correct
termination also prevents Pol II … is depleted from the nucleus with similar kinetics (Figure 1C). …

Single-molecule characterization of Sen1 translocation properties provides insights into eukaryotic factor-dependent transcription termination

S Wang, Z Han, TR Strick - Nucleic Acids Research, 2024 - academic.oup.com
RNA polymerase II (Pol II) complex. We quantify Sen1 translocation activity on single-stranded …
with kinetic competition models for Sen1-dependent termination of transcribing Pol II (6). …

[PDF][PDF] Cell-cycle modulation of transcription termination factor Sen1

HE Mischo, Y Chun, KM Harlen, BM Smalec, S Dhir… - Molecular cell, 2018 - cell.com
… -coding transcripts (ncRNA) generated by RNA polymerase II in S. cerevisiae are terminated
by … nous Sen1 is degraded with somewhat slower kinetics than the plasmid-encoded Sen1 (…

[PDF][PDF] Effects of transcription elongation rate and Xrn2 exonuclease activity on RNA polymerase II termination suggest widespread kinetic competition

N Fong, K Brannan, B Erickson, H Kim, MA Cortazar… - Molecular cell, 2015 - cell.com
… Here we investigate the universality of the torpedo model of pol II termination in human cells
… tested two central predictions of the torpedo model for RNA pol II transcription termination (…

[HTML][HTML] Disengaging polymerase: terminating RNA polymerase II transcription in budding yeast

HE Mischo, NJ Proudfoot - Biochimica et Biophysica Acta (BBA)-Gene …, 2013 - Elsevier
DNA and releases its transcript. Initial scrutiny of protein-coding genes transcribed by RNA
Polymerase II (Pol II), … distinguish between controlled or scheduled transcription termination

A bacterial-like mechanism for transcription termination by the Sen1p helicase in budding yeast

O Porrua, D Libri - Nature structural & molecular biology, 2013 - nature.com
… We demonstrate that Sen1-dependent termination is exclusive for eukaryotic RNAPII and …
Therefore it is possible that transcription and Sen1p translocation compete kinetically and …

Transcription termination and the control of the transcriptome: why, where and how to stop

O Porrua, D Libri - Nature reviews Molecular cell biology, 2015 - nature.com
… , transcription initiation by RNA polymerase II (Pol II) occurs … the various termination pathways
for Pol II-dependent transcription, … to subtract mRNAs from competing nuclear degradation …