Single-molecule FRET method to investigate the dynamics of transcription elongation through the nucleosome by RNA polymerase II

J Lee, JB Crickard, JC Reese, TH Lee - Methods, 2019 - Elsevier
… Data analysis for the kinetics of transcription elongation Upon obtaining FRET time
trajectories from individual ECs, we extract the kinetic rate constants of transcription elongation …

Saccharomyces cerevisiae Sen1 as a Model for the Study of Mutations in Human Senataxin That Elicit Cerebellar Ataxia

X Chen, U Müller, KE Sundling, DA Brow - Genetics, 2014 - academic.oup.com
… for efficient termination of RNA polymerase II transcription of … defects in Sen1-dependent
transcription termination. The … SEN1 mutations on Pol II transcription termination. Functions …

DSIF factor Spt5 coordinates transcription, maturation and exoribonucleolysis of RNA polymerase II transcripts.

K Kus, L Carrique, T Kecman, M Fournier… - bioRxiv, 2023 - biorxiv.org
… considered when assessing their effect on the RNA degradation kinetics of Xrn2. We …
U1 complex of the spliceosome104, which may indicate that there is a competition between

[PDF][PDF] RNA Pol II cleavage activity is a major effector of transcription dynamics

RM Sheridan - 2019 - … .digitalcollections.cuanschutz.edu
… , transcription termination occurs at a position influenced by a kinetic competition between pol
II and termination factor … The mechanisms that influence the release of 5’ paused pol II and …

Common genomic elements promote transcriptional and DNA replication roadblocks

K Roy, J Gabunilas, A Gillespie, D Ngo… - Genome …, 2016 - genome.cshlp.org
… Recent work demonstrated existence of a kinetic competition between Pol II elongation and
… , we reasoned that sites with Pol II readthrough should still roadblock DNA Pol δ. Indeed, we …

RNA quality control in the nucleus: the Angels' share of RNA

O Porrua, D Libri - Biochimica et Biophysica Acta (BBA)-Gene Regulatory …, 2013 - Elsevier
… of Rpb1p, the largest subunit of RNA Pol II (indicated by an arrow). … the nascent RNA and
induces termination of transcription (… Quality control by kinetic competition obviates the need for …

[PDF][PDF] Sen1 and Rrm3 ensure permissive topological conditions for replication termination

R Choudhary, J Niska-Blakie, M Adhil, G Liberi… - Cell Reports, 2023 - cell.com
… , but not rrm3, builds up RNA polymerase II (RNPII) at TERs and telomeres. … We then analyzed
the kinetics of appearance and … Sen1dependent RNPII accumulation compared to rrm3D. …

[HTML][HTML] PAR-CLIP data indicate that Nrd1-Nab3-dependent transcription termination regulates expression of hundreds of protein coding genes in yeast

S Webb, RD Hector, G Kudla, S Granneman - Genome biology, 2014 - Springer
… domain (CTD) of RNA polymerase II. Because this interaction … Our data suggest that
Nrd1-Nab3 can terminate transcription of … by kinetic competition between Nrd1-Nab3 and the CPF …

[HTML][HTML] RNA polymerase II depletion promotes transcription of alternative mRNA species

L Yu, M Rege, CL Peterson, MR Volkert - BMC molecular biology, 2016 - Springer
… sites, whereas enhanced elongation rates favor 3′ end formation at … a kinetic competition
between RNAPII elongation and 3′ end selection. Recently, we reported that transcription of …

Helicases as transcription termination factors: Different solutions for a common problem

Z Han, O Porrua - Transcription, 2018 - Taylor & Francis
termination factors described above, RNA polymerase pausing is a strict requirement for
Sen1-dependent termination … for a kinetic competition between termination and RNAPII …