… In summary, with proper optimisation Luminex … cytokines in mucosal biopsies. Our results
should help other researchers seeking to quantify multiple low concentration cytokines in small …

… however, we did note a small decline in the percentage of CD45RA… Finally, the ELISpot assay
provides quantification of cytokine … high concentrations of bowel microbial flora on mucosal …

… cytokine concentrations were intrinsically associated with sample weight and protein
concentration however CVF cytokines … FGF comprises of cervical mucous/fluid (CF) and vaginal …

… to produce low levels of IL-3 as well as IL-11, cytokines … (TCC) from the small intestinal
mucosa of patients with coeliac … Semiquantitative determination of PCR amplified cytokine …

… Cytokine primers optimized for real-time PCR were designed … Relative quantitation of cytokine
gene expression was … expression in 50 HIV-1–infected subjects showed increases in small …

… The current study investigated adaptability of kits to small tear … medial aspect of the nasal
mucosa. Stimulated samples were … The lower limits of linearity for standard curve determination …

… The 10 cytokine mRNA transcripts quantified in this study were present at different levels …
had a sensitivity which allowed them to detect relatively small amounts of cytokine mRNA. …

… cytokines have only a short half-life (minutes) in serum (1, 6). Therefore, levels of cytokines
are usually low … Quantification of cytokine levels in supernatants of PBMC avoids many of the …

… As a result of an inappropriate activation, the mucosal immune … could find the quantitation
of at least one cytokine/chemokine … We tested whether it was possible to use a relatively small …

… Limitations of the present study are the relatively small sample size and the fact that it is
cross-sectional and therefore does not shed illumination on potential roles for secreted …