… -hydrolyzing mutants of BChE in the dicotyledonous plant Nicotiana benthamiana using the
… that plants may be an attractive means of producing cocaine-hydrolyzing variants of BChE …

… Plants as a source of butyrylcholinesterase variants designed for enhanced cocaine
hydrolase activity. Chem Biol Interact 203, 217–220, doi:10.1016/j.cbi.2012.09.004 (2013). …

… , I demonstrate proof-of-principle that plants can be used as a source to express BChE
variants designed for enhanced cocaine hydrolase activity at quantities relevant to clinical use. …

… of the cocaine metabolites and may even cause vasodilation, … design a cocaine
hydrolase (CocH), ie, a BChE mutant with a considerably improved catalytic activity against (−)-cocaine. …

… bean and cocaine in the leaves of the coca plant are naturally … that BChE with improved
cocaine hydrolase activity could be … The cocaine hydrolase mutant used by Teva was designed …

… Nicotiana benthamiana plant lines expressing a reengineered human butyrylcholinesterase
(BChE) with enhanced cocaine hydrolase activity were created. Subsequent purification …

… efforts have enhanced the cocaine hydrolase (CocH) activity of this … To obtain a form of CocH
that might be developed as a … (1) modified versions of BChE, (2) natural cocaine esterases, …

… high-activity BChE mutants known as cocaine hydrolases [4–10]. One of our designed and
… In addition, it requires a long period of time to generate the transgenic animals or plants. It is …

… design and site-directed mutagenesis transformed human serum recombinant
butyrylcholinesterase (BChE) into a highly efficient cocaine … a plant-produced cocaine-hydrolase …

… cocaine, and its variants with improved cocaine hydrolase (… hydrolysis rate have also
been rational designed to reduce the … The rBChE expressed in plants and goat milk was also …