… the cell biology and protein chemistry of this cytotoxicity. In particular, I introduce … endogenous
granule proteins that are available to regulate lysis by the essential poreforming protein, …

… report that calreticulin inhibits perforin-mediated lysis. The … as the recently purified chymase
(12). To determine whether … calreticulin, an endogenous cytotoxic granule protein, inhibits …

… granule extracts had similar granzyme A, B and chymase … Therefore, the protein concentration
precludes calreticulin as … −/− granule extracts could block perforin-mediated lysis of red …

… the issue of up- vs down-regulation of lysis remains open. … the other endogenous chymases
lacked an effect on lysis under … protein calreticulin is a major constituent of lytic granules in …

… Chymase 1 was previously identified as required for granule-… the effects of calreticulin on
perforin-mediated killing of cells.At … the endogenous calreticulin are equivalent, then the intra-…

… proteins involved in lytic granule release and NK cell-mediated killing of tumor cells. This
review focuses on lytic granules … of granules, presence of calreticulin and calreticulin-mediated …

… cluster in humans also encodes the chymases granzyme H25 and mast-cell chymase (Kcat/Km
= … , which binds to perforin and potentially inhibits perforin-mediated damage.Calreticulin …

… two pathways are complementary, one requiring cell-cell contact and using a regulated ligand
able to lyse … -binding protein calreticulin is a major constituent of lytic granules of cytolytic …

… an endogenous endonuclease or a proximal regulatory protein in … calreticulin has recently
been identified in the granules of … Perforinmediated target cell lysis by cytolytic T lymphocytes. …

… critical for control of tumor development and viral infections. … different species in protein- or
cell-based cytotoxicity assays. For … is critical to induce PS and calreticulin (Crtl) translocation, …