… Immunogold labeling of VHb in both Vitreoscilla and recombinant Escherichia coli bearing
… that the related flavohemoglobin from E. coli is a nitric-oxide dioxygenase that dioxygenates …

New developments have forced a re-evaluation of our understanding of the structure and
function of hemoglobins. Leghemoglobins regulate oxygen affinity through a mechanism …

… of either the artificial flavohemoglobin VHb-Red or of FHP resulted in significant growth
improvements relative to the native VHb protein in microaerobic growing Escherichia coli cells. …

… coli flavohemoglobin (Hmp). The locations of the respective … by an exogenous water ligand
in the distal position. Because … sustained NO reductase or oxygenase (denitrosylase) activity. …

… ], likely through water molecules [9], and two residues on helix E (… distal residue to the heme
iron in the flavohemoglobin of E. … it is relevant for the NO-dioxygenase reaction and it was not …

… (22) showed that the substitution of Tyr(B10) for a Phe in E. coli flavoHb decreases the NOD
… does occur from time to time (leading to a loss of function), it appears that trHbN prevents its …

… flavohemoglobin Fhp was found to be able to relieve nitrosative stress in Escherichia coli. It
… the isomerization of a putative peroxynitrite intermediate in the NO dioxygenase reaction ( …

… bioremediation, a possible role in enhancing aerobic waste water treatment, and … dioxygenase
(NOD) VHb closely resembles the heme domain of bacterial and yeast flavohemoglobins …

… domains, namely a rubredoxin domain in the Escherichia coli protein and a NAD(P)… coli,
respectively, flavohemoglobin and flavorubredoxin, the ytfE gene has been assigned a function …

… flavohemoglobins function in vivo as denitrosylases (O 2 nitroxylases), and some multimeric,
invertebrate hemoglobins function … Therefore, FHb/Hmp functions as an NO dioxygenase …