Topography and refractometry of sperm cells using spatial light interference microscopy

L Liu, ME Kandel, M Rubessa… - … of biomedical optics, 2018 - spiedigitallibrary.org
… that spatial light interference microscopy (SLIM), 6 – 8 a highly sensitive quantitative-phase
imaging (QPI) method, can be used to study unlabeled sperm cells in great detail. QPI is an …

Morphometric analysis of sperm used for IVP by three different separation methods with spatial light interference microscopy

M Rubessa, ME Kandel, S Schreiber… - Systems Biology in …, 2020 - Taylor & Francis
… chose spatial light interference microscopy (SLIM), which is highly sensitive in both space and
… SLIM has been used recently to characterized the topography and refractometry of sperm (…

Topography and refractometry of sperm cells using SLIM

L Liu, ME Kandel, M Rubessa, S Schreiber, M Wheeler… - bioRxiv, 2017 - biorxiv.org
spatial light interference microscopy (SLIM) to decouple the thickness and refractive index
information of individual cells. … cells on media of two different refractive indices and using a …

Spatial light interference microscopy: principle and applications to biomedicine

X Chen, ME Kandel, G Popescu - Advances in optics and photonics, 2021 - opg.optica.org
… the spatial accuracy of SLIM and atomic force microscopy (AFM) by imaging an amorphous
carbon film deposited on glass. The topography … of the sperm cell yield high refractive-index v…

Digital holographic microscopy for the evaluation of human sperm structure

G Coppola, G Di Caprio, M Wilding, P Ferraro… - Zygote, 2014 - cambridge.org
light source, the thickness and the integral refractive index of … obtained by contrast interference
microscopy (phase contrast … (x,y) incorporates information about the topographic profile of …

Quantitative label-free technique for morphological evaluation of human sperm—a promising tool in semen evaluation

PK Poola, V Jayaraman, K Chaithanya, D Rao… - OSA …, 2018 - opg.optica.org
… brightfield microscope and further sorting of cells according to … is the constant refractive index
of 1.35 [18]. This assumption … and un-stained cells using a brightfield microscope. In most of …

[HTML][HTML] Partially spatially coherent digital holographic microscopy and machine learning for quantitative analysis of human spermatozoa under oxidative stress …

V Dubey, D Popova, A Ahmad, G Acharya, P Basnet… - Scientific reports, 2019 - nature.com
… very low refractive index contrast with respect to the medium like tail of the sperm cells. …
investigation thus holography provides a 3D topographic profile of the specimen. The phase …

Quantitative label-free animal sperm imaging by means of digital holographic microscopy

G Di Caprio, MA Gioffre, N Saffioti… - IEEE Journal of …, 2010 - ieeexplore.ieee.org
… to generate a topographical map of the spermatozoa. … sperm cells, as reported in [5], ie,
0.50 µm. From this information, we calculated the value of the refractive index when the OPD is in …

[HTML][HTML] 3D dynamic observation of human sperm by parallel phase-shifting digital holographic microscopy based on pixelated polarization

C Bai, Z Jiang, J Zhao, S Wu, Q Zhang - APL Photonics, 2023 - pubs.aip.org
… -magnification microscope objective, while the sperm flagella (… Microscopic dynamic
observation of intact sperm in 3D is … -dimensional refractive-index profile of the sperm head and …

[HTML][HTML] … spermatozoa substances distribution and morphological differences between Holstein and Korean native cattle using three-dimensional refractive index …

H Jiang, J Kwon, S Lee, YJ Jo, S Namgoong, X Yao… - Scientific reports, 2019 - nature.com
is defined as the integration of the refractive index (RI) along an optical path; the topographic
information (optical thickness) of the sample is … stain-free interferometric phase microscopy