[PDF][PDF] 外切核酸酶Ⅲ 介导的DNA 分子克隆

谢昌传, 梁耀极, 洪丽欣 - 中国生物化学与分子生物学报, 2016 - core.ac.uk
… of DNA ligase,we designated it as ligation-independent cloning ( LIC) . We optimized the …
independent cloning method suitable for high-throughput expression screening applications…

[PDF][PDF] 同源区段长度对In-Fusion 技术连接效率的影响

李慧仙, 朱平 - 中国医药生物技术, 2013 - cmbp.net.cn
… recombinant proteins: marrying ligation independent cloning and in vitro … the cloning vector,
and to establish an efficient high-throughput cloning method based on In-Fusion technique. …

[PDF][PDF] 无限制克隆技术研究进展及其应用

杨传辉, 卢荣锐, 杨愈丰 - Progress in Biochemistry and …, 2019 - researchgate.net
… - and ligationindependent cloning as a rapid and versatile cloning method for functional …
destination vectors for high-throughput cloning and expression screening in Escherichia coli. …

[PDF][PDF] 人神经生长因子β 亚基慢病毒载体的构建*★

苏一鸣, 朱绍兴, 蔡鹏 - Journal of Clinical Rehabilitative Tissue …, 2011 - cjter.com
… Lentiviral vector is an ideal vector for gene therapy. OBJECTIVE: To construct lentiviral …
The precise engineering of expression vectors using high-throughput In-Fusion PCR cloning. …