… A SYBR Green I-based quantitative RT-PCR (qPCR) method was developed for detecting …
Result The newly developed qPCR assay showed a minimum detection limit of 1×10 −1 pg·μL …

… method for the rapid detection of Porcine astrovirus 4 (PAstV4), a pair of specific primer was
… a standard, was detected by SYBR Green Ⅰ fluorescence quantitative RT-PCR assay. And …

… 目前针对该病毒的检测方法包括病毒分离,间接ELISA,普通的PCR和荧光定量RT-PCR,血清中
和… 性的引物和探针,建立了基于TaqMan的实时RT-PCR检测方法.该方法过于昂贵,且需要特殊的…

… development, and the specificity, sensitivity and reproducibility of the assay were evaluated.
… A rapid and sensitive one step-SYBR Green based semi quantitative Real time RT-PCR for …

… detection and evaluation of dengue virus in a mouse model. [Keywords] dngue feverꎻ
mouse modelꎻ real time RT-PCRꎻ TaqMan MGBꎻ quantitative detection … Development and …

… EvaGreen real-time PCR assay was successfully developed … 近年来,RT-PCR方法已成为诊断
BNoV感染的主要手段,且BNoV… Detection and evolutionary analysis of bovine noroviruses in …

… A TaqMan RT-PCR method was established for detecting … to 2018, the newly developed
method showed a high prevalence of … Cloning and sequence analysis of ORF 3 gene of porcine …

… real time quantitative PCR, competitive PCR, serial analysis of … real time RT PCR[ J] . Int J
Cancer, 2005, 114( 2) : 174 181 … deter mined by SYBR Green based quantitative realtime PCR[ …

… -PCR) assays have been developed for detecting different … Our phylogenetic analysis of
complete fusion protein gene of … the NP-gene rRT-PCR and fusion gene RT-PCR assay ------------…

… (TBRI) had already developed several resistant banana cultivars … could be detected in both
of them with RT-PCR assays. … in cardamom using SYBR Green based reverse transcription-…