建立反轉錄病毒感染小鼠初級T 細胞的基因轉殖系統

江偉微 - 臺北醫學大學細胞及分子生物研究所學位論文, 2001 - airitilibrary.com
… to produce high titer recombinant retrovirus. PhoenixE cell line carrying plasmids encoding
retroviral … was used to produce recombinant virus after transfecting viral vector which contains …

[HTML][HTML] 利用MigR1 质粒构建CD19-CAR 逆转录病毒载体优化其对人原代T 细胞的转染效率

王扬, 唐古生, 徐丽丽, 阮婕, 程辉, 周虹… - Chinese Journal of …, 2015 - ncbi.nlm.nih.gov
retroviral vector to produce the high titer retrovirus. ②MigR1-CD19-CAR transduction efficiency
of K562 cell line … a second generation CAR which targeted to CD19 through a retroviral

[HTML][HTML] 中试制备临床级别第三代慢病毒

段德明, 季永飘, 周梦洁, 高基民 - 生物工程学报, 2019 - cjb.ijournals.cn
… to the third generation in terms of safety. The preparation of lentiviruses with high titer
used as an HEK293T cell carrier matrix, and several sterile cell culture spinners were combined …

慢病毒介导可溶性肿瘤坏死因子受体1 在小鼠骨髓未成熟树突状细胞中的表达

黄一虹, 晁亚丽, 汤仁仙, 王淑华, 曾令宇, 陈翀… - 中国组织工程研究, 2010 - cjter.com
vectors carrying sTNFR1 gene and eGFP reporter gene were successfully constructed, and
recombinant lentiviral plasmids with high titer … dendritic cells through the use of viral vector

[HTML][HTML] CAR-T 细胞治疗产品中复制型病毒的风险分析及控制

吴雪伶, 赵翔, 孟淑芳 - 中国药事, 2018 - zgys.cnjournals.org
… At present, retroviral vectors or lentiviral vectors are … T cells in the production of CAR-T cell
products. However, the use of these vectors may cause the risk of contamination of CAR-T cell

血纖維蛋白溶酶原片段的抗血管新生活性及其基因治療效果

林銘德 - 2002 - nckur.lib.ncku.edu.tw
… Male B6 mice 6 to 8 week-old were divided into various groups depending on the number
of prepared retroviral vectors. Mice were injected subcutaneously with 1x 10 5 B16 F10 cells. …

[PDF][PDF] 蛋-----Renilla Luc (siCont)

N Sago - mhlw-grants.niph.go.jp
… Transduction of dendritic cells by DNA viral vectors directs the immune response to transgene
… Feasibility of generating adeno-associated virus packaging cell lines containing inducible …

[HTML][HTML] 新型重组AAV5/5 载体及包装系统

董小岩, 田文洪, 袁振华, 谭淑萍, 吴小兵 - 生物工程学报, 2010 - m.fx361.com
… EGFP expression positive monoclonal cells were picked up, and one that produced rAAV5/5…
production cell line named as C020. rAAV5/5-EGFP was produced by infecting C020 cells

[PDF][PDF] HIV-1 载体的研发沿革

张帆, 张敬之 - Chinese Bulletin of Life Sciences, 2010 - lifescience.sinh.ac.cn
retroviral vectors, such as murine leukemia virus (MLV) vector, or foamy virus(FV) vector,
HIV-1 vector … A stable human-derived packaging cell line for production of high titer retrovirus/…

rAAV/ABAD-DP-6His 可有效减轻PC12 细胞的氧化应激损伤

M Jia, M Wang, Y Yang, Y Chen, D Liu… - 中国神经再生研究 …, 2014 - sjzsyj.com.cn
… The cell culture medium was then removed, and 100 μL of … and was harvested at a high
titer. We sought to establish a … + overload can lead to enhanced generation of ROS, triggering …