… 增加而增加,PCR-DGGE 技术在评价活性污泥系统中微生物群落结构的变化 方面方便快捷,具有
良好的应用前景[22];2) 巢式PCRDGGE/TGGE 技术:利用两套PCR 引物进行两轮PCR 扩增反应,…

… measured and diluted at a ratio of 1 : 10, and underwent quantitative amplification in the PCR
reaction system as described above. In this study, we used a relatively quantitative method …

… 对PCR 产物直接测序将导致无法读出其序 列,必须对PCR 产物进行常规克隆后再测序,如杉 科密
叶杉属植物Athrotaxis laxifolia 和A. cupressoides 的ITS1 序列,如果PCR … 直接测定PCR 产物的…

… The reagents of RACE and PCR were using Clontech and Qiagen protocols. Restriction
endonucleases and T4 DNA ligase were from NEB. pMD18-T vector was bought from TaKaRa. …

Ammonia monooxygenase (AMO) is one of the key enzymes related to nitrification process,
and the corresponding gene is amo. In this study, two pairs of primers were designed …

… Genomic DNA Kit and polymerase chain reaction (PCR) amplification of CKIT DNA … PCR
amplified fragments of CKIT gene exons 921 were sequenced according to device instructions…

… Referring to the method design in the literature, 4 we synthesized a 3.0kilobase (kb)
promoter at the 5' flanking region of LRP16 and polymerase chain reaction (PCR) primers for …

… Abstract: [Objective]The objective of this study is to develop a rapid triplex PCR detection
method of Corynespora cassiicola,Colletotrichum orbiculare and Pseudomonas syringae pv. …

… -time PCR method, the method was improved, and reference ability verification guidance
book … Results Rapid screening was carried out by the real-time PCR method to preliminarily …