基于荧光素酶生物发光检测方法的研究及其应用进展
刘琳, 胡婷婷, 王梦灵, 聂耀, 张惟杰, 王琛… - 中国药科大学 …, 2023 - jcpu.cpu.edu.cn
生物发光现象广泛存在于自然界中, 不管是在陆地或是海洋都有发光生物的踪迹.
其中基于荧光素酶的生物发光系统被广泛研究, 启发着人类在基因, 表观遗传等方面进行探索 …
其中基于荧光素酶的生物发光系统被广泛研究, 启发着人类在基因, 表观遗传等方面进行探索 …
Advance on detection method and application based on bioluminescence of luciferase
L LIU, T HU, M WANG, Y NIE… - Journal of China …, 2023 - pesquisa.bvsalud.org
ABSTRACT@# Bioluminescence is a widespread phenomenon in nature, and luminescent
organisms can be found both on land and in the ocean. Among them, luciferase based …
organisms can be found both on land and in the ocean. Among them, luciferase based …
Current advanced bioluminescence technology in drug discovery
H Hoshino - Expert Opinion on Drug Discovery, 2009 - Taylor & Francis
Background: Bioluminescence technology is based on the luciferin–luciferase reaction and
is generally well known as a reporter gene assay system that uses firefly luciferase. It has …
is generally well known as a reporter gene assay system that uses firefly luciferase. It has …
Bioluminescence assays: multicolor luciferase assay, secreted luciferase assay and imaging luciferase assay
Y Nakajima, Y Ohmiya - Expert opinion on drug discovery, 2010 - Taylor & Francis
Importance of the field: By selecting the most appropriate bioluminescence assay, the
researcher can study the underlying molecular mechanisms of a physiological system and …
researcher can study the underlying molecular mechanisms of a physiological system and …
Application of enzyme bioluminescence for medical diagnostics
LA Frank, VV Krasitskaya - … and Applications in Biotechnology-Volume 1, 2014 - Springer
<? xm-replace_text {Heading}?> Abstract Nowadays luciferases are effectively used as
analytical instruments in a great variety of research fields. Of special interest are the studies …
analytical instruments in a great variety of research fields. Of special interest are the studies …
Novel functional Renilla luciferase mutant provides long-term serum stability and high luminescence activity
Fluorescent and luminescent chemical probes are essential in recent medical diagnostics.
However, the use of these probes in vivo has raised concerns due to their low sensitivity …
However, the use of these probes in vivo has raised concerns due to their low sensitivity …
Practical application of bioluminescence enzyme immunoassay using enhancer for firefly luciferin–luciferase bioluminescence
T Minekawa, H Ohkuma, K Abe, H Maekawa… - …, 2011 - Wiley Online Library
Firefly luciferin–luciferase bioluminescence is known for its high quantum yield (41.0±7.4%).
Given this high quantum yield, application of this bioluminescence is expected to be useful …
Given this high quantum yield, application of this bioluminescence is expected to be useful …
Activity‐Based Luciferase‐Luciferin Bioluminescence System for Bioimaging Applications
T Xia, X Cheng, W Zhan, G Liang - Analysis & Sensing, 2021 - Wiley Online Library
There is growing interest in applying bioluminescence for imaging varies biological
processes in life sciences due to its high resolution, selectivity, and signal/noise ratio (no …
processes in life sciences due to its high resolution, selectivity, and signal/noise ratio (no …
1001 lights: luciferins, luciferases, their mechanisms of action and applications in chemical analysis, biology and medicine
ZM Kaskova, AS Tsarkova, IV Yampolsky - Chemical Society Reviews, 2016 - pubs.rsc.org
Bioluminescence (BL) is a spectacular phenomenon involving light emission by live
organisms. It is caused by the oxidation of a small organic molecule, luciferin, with molecular …
organisms. It is caused by the oxidation of a small organic molecule, luciferin, with molecular …
Applications of luciferin biosynthesis: bioluminescence assays for l-cysteine and luciferase
K Niwa, Y Nakajima, Y Ohmiya - Analytical biochemistry, 2010 - Elsevier
Based on the biosynthetic pathway of the firefly bioluminescence substrate d-luciferin, the
concentration of l-cysteine can be quantified using a simple protocol and a conventional …
concentration of l-cysteine can be quantified using a simple protocol and a conventional …