基于荧光素酶生物发光检测方法的研究及其应用进展

刘琳, 胡婷婷, 王梦灵, 聂耀, 张惟杰, 王琛… - 中国药科大学 …, 2023 - jcpu.cpu.edu.cn
生物发光现象广泛存在于自然界中, 不管是在陆地或是海洋都有发光生物的踪迹.
其中基于荧光素酶的生物发光系统被广泛研究, 启发着人类在基因, 表观遗传等方面进行探索 …

Advance on detection method and application based on bioluminescence of luciferase

L LIU, T HU, M WANG, Y NIE… - Journal of China …, 2023 - pesquisa.bvsalud.org
ABSTRACT@# Bioluminescence is a widespread phenomenon in nature, and luminescent
organisms can be found both on land and in the ocean. Among them, luciferase based …

Current advanced bioluminescence technology in drug discovery

H Hoshino - Expert Opinion on Drug Discovery, 2009 - Taylor & Francis
Background: Bioluminescence technology is based on the luciferin–luciferase reaction and
is generally well known as a reporter gene assay system that uses firefly luciferase. It has …

Bioluminescence assays: multicolor luciferase assay, secreted luciferase assay and imaging luciferase assay

Y Nakajima, Y Ohmiya - Expert opinion on drug discovery, 2010 - Taylor & Francis
Importance of the field: By selecting the most appropriate bioluminescence assay, the
researcher can study the underlying molecular mechanisms of a physiological system and …

Application of enzyme bioluminescence for medical diagnostics

LA Frank, VV Krasitskaya - … and Applications in Biotechnology-Volume 1, 2014 - Springer
<? xm-replace_text {Heading}?> Abstract Nowadays luciferases are effectively used as
analytical instruments in a great variety of research fields. Of special interest are the studies …

Novel functional Renilla luciferase mutant provides long-term serum stability and high luminescence activity

WC Song, HJ Sung, KS Park, JW Choi, JY Cho… - Protein Expression and …, 2013 - Elsevier
Fluorescent and luminescent chemical probes are essential in recent medical diagnostics.
However, the use of these probes in vivo has raised concerns due to their low sensitivity …

Practical application of bioluminescence enzyme immunoassay using enhancer for firefly luciferin–luciferase bioluminescence

T Minekawa, H Ohkuma, K Abe, H Maekawa… - …, 2011 - Wiley Online Library
Firefly luciferin–luciferase bioluminescence is known for its high quantum yield (41.0±7.4%).
Given this high quantum yield, application of this bioluminescence is expected to be useful …

Activity‐Based Luciferase‐Luciferin Bioluminescence System for Bioimaging Applications

T Xia, X Cheng, W Zhan, G Liang - Analysis & Sensing, 2021 - Wiley Online Library
There is growing interest in applying bioluminescence for imaging varies biological
processes in life sciences due to its high resolution, selectivity, and signal/noise ratio (no …

1001 lights: luciferins, luciferases, their mechanisms of action and applications in chemical analysis, biology and medicine

ZM Kaskova, AS Tsarkova, IV Yampolsky - Chemical Society Reviews, 2016 - pubs.rsc.org
Bioluminescence (BL) is a spectacular phenomenon involving light emission by live
organisms. It is caused by the oxidation of a small organic molecule, luciferin, with molecular …

Applications of luciferin biosynthesis: bioluminescence assays for l-cysteine and luciferase

K Niwa, Y Nakajima, Y Ohmiya - Analytical biochemistry, 2010 - Elsevier
Based on the biosynthetic pathway of the firefly bioluminescence substrate d-luciferin, the
concentration of l-cysteine can be quantified using a simple protocol and a conventional …