The Cost-Efficiency Realization in the Escherichia coli-Based Cell-Free Protein Synthesis Systems
Q Lian, H Cao, F Wang - Applied biochemistry and biotechnology, 2014 - Springer
A reliable and cost-efficient protein synthesis system is the prerequisite for both structural
and functional proteomic studies. The last decades saw the great technological …
and functional proteomic studies. The last decades saw the great technological …
Rational design and applications of industrial proteins
G Qu, B Yuan, Z Sun - Sheng wu Gong Cheng xue bao= Chinese …, 2022 - europepmc.org
As one of the underlying core technologies in the fields of synthetic biology and green bio-
manufacturing, rational protein design is able to effectively solve generic challenges, eg …
manufacturing, rational protein design is able to effectively solve generic challenges, eg …
基于分子机制的蛋白质酶解反应经验修饰动力学模型
齐崴， 何志敏 - 2006 - jmcchina.org
蛋白质酶解反应动力学行为的复杂性在于体系中底物与产物的多样性, 以及由此决定的动力学
常数的可变性. 基于此, 以酪蛋白(case in)—胰蛋白酶(trypsin) 为模式体系, 拟合求得动力学常数 …
常数的可变性. 基于此, 以酪蛋白(case in)—胰蛋白酶(trypsin) 为模式体系, 拟合求得动力学常数 …
Improvement of enzymatic stability and catalytic efficiency of recombinant Fusariumoxysporum trypsin with different N-terminal residues produced by Pichiapastoris
N Yang, Z Ling, L Peng, Y Liu, P Liu… - … of Microbiology and …, 2018 - koreascience.kr
Fusarium oxysporum trypsin (FOT) is a fungal serine protease similar to mammal trypsin.
The FOT could be successfully expressed in Pichiapastoris by engineering the natural …
The FOT could be successfully expressed in Pichiapastoris by engineering the natural …
酶的分子设计, 改造与工程应用
朱俊晨， 王小菁 - 中国生物工程杂志, 2004 - manu60.magtech.com.cn
酶工程的研究已经发展到分子水平, 在体外通过基因工程, 化学, 物理等手段改造酶分子结构与
功能, 大幅提高了酶分子的进化效率和催化效率, 生产有价值的非天然酶. 对酶工程学若干“热点” …
功能, 大幅提高了酶分子的进化效率和催化效率, 生产有价值的非天然酶. 对酶工程学若干“热点” …
[HTML][HTML] 工业蛋白质理性设计与应用
曲戈， 袁波， 孙周通 - 生物工程学报, 2022 - cjb.ijournals.cn
作为合成生物学与绿色生物制造等领域的底层核心技术, 蛋白理性设计可有效解决天然功能元件
性能不足等共性挑战, 创制高性能人工酶元件. 值此天津工业生物研究所(Tianjin Institute of …
性能不足等共性挑战, 创制高性能人工酶元件. 值此天津工业生物研究所(Tianjin Institute of …