丝状真菌目标基因替换过程中的策略与方法

王教瑜, 杜新法, 柴荣耀, 孙国昌, 林福呈 - 遗传, 2007 - chinagene.cn
… In this paper, strategies involved in TGR in filamentous fungi were reviewed, including …
Efficient gene identification and targeted gene disruption in the wheat blotch fungus

[PDF][PDF] GUS 为负标记的稻瘟病菌目标基因替换突变体双筛选体系

王教瑜, 张震, 杜新法, 柴荣耀, 毛雪琴, 邱海萍… - 生物工程学报, 2009 - Citeseer
… years, this procedure has been used to identify and characterize the genes in filamentous
fungi, a … , where the target fungus transformed with a replacement vector yields two kinds of …

丝状真菌中的RNA 干扰及其应用技术

王绍文, 刘刚, 邢苗, 田生礼 - 生物技术通报, 2011 - biotech.aiijournal.com
filamentous fungi. It has special significance to employ RNA interference as a tool for molecular
biology research and genetic manipulation of filamentous fungi,… in filamentous fungi. Key …

CRISPR-Cas 系统编辑丝状真菌的进展与挑战

肖晗, 刘宜欣 - 合成生物学, 2021 - synbioj.cip.com.cn
… -Cas system in gene editing for filamentous fungi that has been … 来源于构巢曲 霉(Aspergillus
nidulans)的AMA1 序列是迄今为 … and bipartite gene targeting substrates, as effective gene

[HTML][HTML] 黑曲霉作为细胞工厂: 知识准备与技术基础

郭艳梅, 郑平, 孙际宾 - 生物工程学报, 2010 - fx361.com
… 基因替换的是构巢曲霉Aspergillus nidulans (Eidam) Winter,烟… Highly efficient gene
replacements in Neurospora strains … -secretion system in filamentous fungi. Chin J Biotech, 2002, …

工业丝状真菌基因组编辑技术研究进展

刘倩, 李金根, 张晨阳, 李芳雅, 田朝光 - 合成生物学, 2021 - synbioj.cip.com.cn
efficiency of homologous integration in filamentous fungi is very low using classical genetic
… 例如,粗糙脉孢菌(Neurospora crassa)和构巢曲 霉(Aspergillus nidulans)等被作为模式真菌用…

十字花科黑斑病菌生合成tenuazonic acid 之PKS-NRPS 基因鑑定

賴羿璇 - 2013 - tdr.lib.ntu.edu.tw
… system on Aspergillus nidulans LO2026 strain in order to find out the role that AB04556.1
gene played in the biosynthesis of TA. In this study, we found a potentially candidate gene from …

CRISPR/Cas9 系统在丝状真菌中的研究进展

王佩, 刘文, 沈祥陵 - 生物技术通报, 2018 - biotech.aiijournal.com
… in filamentous fungi,this paper introduced various editing mechanisms in filamentous fungi,
… 结果显 示该体系成功的对构巢曲霉(Aspergillus nidulans), 黑曲霉(Aspergillus niger),棘孢曲霉(…

CRISPR/Cas 基因编辑及其新兴技术在丝状真菌研究中的系统应用

陈盈盈, 刘扬, 史俊杰, 马俊英, 鞠建华 - 合成生物学, 2024 - synbioj.cip.com.cn
gene editing systems. In general, this review will provide useful guidance for developing an
efficient gene editing platform in various filamentous fungiAspergillus nidulans in the post-…

[HTML][HTML] 基于CRISPR/Cas 系统的黑曲霉基因组编辑技术

郑小梅, 郑平, 孙际宾 - 生物工程学报, 2021 - cjb.ijournals.cn
… ,最早Nodvig等 [8] 利用来自于构巢曲霉Aspergillus nidulans的RNA聚合酶Ⅱ所识别的强
启动子… /Cas9 technology in filamentous fungi. Computat Struct Biotechnol J, 2019, 17: 761-769. …