An aptly positioned azido group in the spacer of a protein cross‐linker for facile mapping of lysines in close proximity
PT Kasper, JW Back, M Vitale, AF Hartog… - …, 2007 - Wiley Online Library
… protein cross-linker explored in this study was based on a reaction recently discovered in our
group, namely the azide… occurs, along with reduction of the azide without cleavage.33 We …
group, namely the azide… occurs, along with reduction of the azide without cleavage.33 We …
Development of a novel cross-linking strategy for fast and accurate identification of cross-linked peptides of protein complexes
… -cleavable cross-linker for the characterization of a multisubunit protein … aptly positioned
azido group in the spacer of a protein cross-linker for facile mapping of lysines in close proximity…
azido group in the spacer of a protein cross-linker for facile mapping of lysines in close proximity…
[HTML][HTML] The beginning of a beautiful friendship: cross-linking/mass spectrometry and modelling of proteins and multi-protein complexes
J Rappsilber - Journal of structural biology, 2011 - Elsevier
… acid pairs that are positioned in close proximity to each other. … rest of the cross-linker or
may contain another protein, if the … the length of the spacer in the cross-linker and the length of …
may contain another protein, if the … the length of the spacer in the cross-linker and the length of …
Divide and conquer: cleavable cross-linkers to study protein conformation and protein–protein interactions
A Sinz - Analytical and bioanalytical chemistry, 2017 - Springer
… Also, hydrophobic protein regions lacking lysines are not … in the peptide map after reduction
of the cross-linker. This … reducing agent: (i) The azide in the spacer of BAMG is reduced to an …
of the cross-linker. This … reducing agent: (i) The azide in the spacer of BAMG is reduced to an …
A new in vivo cross-linking mass spectrometry platform to define protein–protein interactions in living cells
RM Kaake, X Wang, A Burke, C Yu, W Kandur… - Molecular & Cellular …, 2014 - ASBMB
… , and MS-cleavable cross-linker with multistage tandem mass … mapping PPIs and their
interaction interfaces in living cells is critical not only for a comprehensive understanding of protein …
interaction interfaces in living cells is critical not only for a comprehensive understanding of protein …
Selective enrichment and identification of cross-linked peptides to study 3-D structures of protein complexes by mass spectrometry
H Buncherd, MA Nessen, N Nouse, SK Stelder… - Journal of …, 2012 - Elsevier
… of the protein or protein complex can be placed within the … From the length of the spacer of
the cross-linker, the … of the cross-linker used in our experiments enabled mapping of several …
the cross-linker, the … of the cross-linker used in our experiments enabled mapping of several …
Cross-linking mass spectrometry for mapping protein complex topologies in situ
K Lee, FJ O'Reilly - Essays in Biochemistry, 2023 - portlandpress.com
… , the cross-linker may require additional functionality in its spacer … are highly reactive with
lysines and the N-termini of proteins, with … model that placed NusA into the unexplained density. …
lysines and the N-termini of proteins, with … model that placed NusA into the unexplained density. …
Selective enrichment and identification of azide-tagged cross-linked peptides using chemical ligation and mass spectrometry
D Vellucci, A Kao, RM Kaake… - Journal of the …, 2010 - ACS Publications
… method not only for mapping protein-protein interactions of all … Our cross-linker targets lysine
residues and the free protein N… expanded azide-DSG (7.7 Å spacer length) and two lysine …
residues and the free protein N… expanded azide-DSG (7.7 Å spacer length) and two lysine …
Development of large-scale cross-linking mass spectrometry
HM Barysz, J Malmström - Molecular & Cellular Proteomics, 2018 - ASBMB
… a detailed linkage map, when isolated proteins are analyzed, … the cross-linker has reacted
with residues on both its ends, … bond joining the lysine ε-amine with the cross-linker or at the …
with residues on both its ends, … bond joining the lysine ε-amine with the cross-linker or at the …
In-culture cross-linking of bacterial cells reveals large-scale dynamic protein–protein interactions at the peptide level
L De Jong, EA De Koning, W Roseboom… - Journal of proteome …, 2017 - ACS Publications
… change, we added the cross-linker directly in the culture … in which the azido group in the
spacer of the cross-linker has … score also placed δ in this region, but the remaining eight placed it …
spacer of the cross-linker has … score also placed δ in this region, but the remaining eight placed it …