包涵体蛋白的分离和色谱法体外复性纯化研究进展

王增, 马会勤, 张文, 陈尚武 - 中国生物工程杂志, 2009 - manu60.magtech.com.cn
Refolding of protein inclusion bodies directly … inclusion body solubilization and refolding
at large scale using expanded-bed adsorption chromatography from Escherichia coli. Protein

包涵体溶解与复性的策略与方法

王苹, 井健 - Bioprocess, 2012 - hanspub.org
… denatured protein molecules in the form of particles. Refolding of inclusion body proteins
into … proteins from E. coli. With new information available on the structure and function of …

包涵体复性研究进展(英文)

龚平生, 罗贵民 - 中国生物工程杂志, 2003 - manu60.magtech.com.cn
body expression may be the best available production method. The challenge is to take …
inclusion body proteins by being able to convert inactive and misfolded inclusion body proteins

[PDF][PDF] 大肠杆菌中同时表达的可溶性和不溶性人α-干扰素-2b 的初步分离研究

徐志南, 岑沛霖 - 中国化学工程学报, 2002 - cjche.cip.com.cn
… For soluble IFN sample, the process of inclusion body solubilization and protein refolding is
… For insoluble IFN sample, the insoluble inclusion body had to be solubilized, refolded and …

人血管内皮生长因子及其受体在大肠杆菌中的克隆, 表达, 纯化及功能研究

刘立成 - 2009 - ir.lzu.edu.cn
… The traditional protein purification method contais inclusion bodies cleaning ,inclusion
bodies solubilization ,proteins purification and refolding.Our current work provides a novel …

[引用][C] 显著提高大肠杆菌中外源基因表达产物可溶性的双顺反子表达载体pNJUTRX-1 (英文)

杨杉, 赵景晖, 华子春 - 南京大学学报: 自然科学版, 2000 - cqvip.com
solubility of target protein hCsx, avoiding the formation of the troublesome inclusion bodies 2
The solubility—improved target protein is … lysozyme [71 to help the folding of target proteins

[PDF][PDF] 卵巢癌抗独特型单链抗体6B11ScFv/鼠HSP70 融合蛋白复性条件的研究

张果, 昌晓红, 成夜霞, 叶雪, 程洪艳, 魏丽惠… - 中国医药生物 …, 2009 - cmbp.net.cn
… of active proteins through refolding of bacterial inclusion body proteins. Microb … refolding
conditions for the 6B11ScFv/mHSP70 fusion protein. The inclusion body of the fusion protein

[HTML][HTML] 青海血蜱Hq012基因在大肠埃希菌中的表达与纯化

马婧, 陈开廷, 广慧, 赵文斌, 杨寅冉, 高金亮… - 中国媒介生物学及控制 …, 2023 - html.rhhz.net
… first refolding followed by purification was an optimal purification method for the tick-derived …
protein rHq012. The study provides a reference for the purification of inclusion body protein. …

结核分枝杆菌Rv0357c 蛋白的生物信息学分析与鉴定

唐王刚, 梅传忠, 高佳慧, 司雨, 连超群 - 生物学杂志, 2021 - swxzz.com
… Rv0357c fusion protein was found to be expressed as inclusion bodies with … solubilizing
the inclusion bodies, affinity chromatography and subsequent protein refolding with a refolding

[PDF][PDF] 豆豉纤溶酶基因在大肠杆菌中的可溶性表达及纯化

张少平, 陈亮, 郭勇 - 生命科学研究, 2010 - smkx.hunnu.edu.cn
proteins,increase the fold鄄 ing of denatured proteins and enhance the solubility of fusion
proteins… to overcome the obstacle of refolding insoluble inclusion bodies. Initial growth of the …