[HTML][HTML] 活细胞RNA 成像技术及其在生物医学中应用研究进展
SUN Pingping, ZOU Wei - Journal of Zhejiang University (Medical …, 2022 - ncbi.nlm.nih.gov
Abstract RNA 可与各种核酸及蛋白质相互作用， 在多种生理和病理过程中发挥重要的调控作用
， 其生物学过程高度动态。 追踪活细胞中的RNA 动态对理解基因表达的时空调控以及RNA …
， 其生物学过程高度动态。 追踪活细胞中的RNA 动态对理解基因表达的时空调控以及RNA …
Research progress of live-cell RNA imaging techniques.
P Sun, W Zou - Zhejiang da xue xue bao. Yi xue ban= Journal of …, 2022 - europepmc.org
RNA molecules play diverse roles in many physiological and pathological processes as they
interact with various nucleic acids and proteins. The various biological processes of RNA …
interact with various nucleic acids and proteins. The various biological processes of RNA …
[引用][C] 活细胞RNA 成像技术及其在生物医学中应用研究进展
孙萍萍， 邹炜 - 浙江大学学报(医学版), 2022 - europepmc.org
RNA 可与各种核酸及蛋白质相互作用, 在多种生理和病理过程中发挥重要的调控作用,
其生物学过程高度动态. 追踪活细胞中的RNA 动态对理解基因表达的时空调控以及RNA …
其生物学过程高度动态. 追踪活细胞中的RNA 动态对理解基因表达的时空调控以及RNA …
[PDF][PDF] 细胞RNA 影像解析技术的研究进展
陈显军， 左方婷， 谢鑫， 方梦悦， 杨弋 - 生命科学, 2021 - lifescience.sinh.ac.cn
RNA 具有独特的三维结构和种类繁多的生物学功能, 广泛参与细胞的各类生命活动,
与多种疾病的发生与发展密切相关. 长期以来, 科学家们一直在追求可以对细胞中RNA …
与多种疾病的发生与发展密切相关. 长期以来, 科学家们一直在追求可以对细胞中RNA …
Fluorescent probes for live-cell RNA detection
Commonly used techniques for analyzing gene expression, such as polymerase chain
reaction (PCR), microarrays, and in situ hybridization, have proven invaluable in …
reaction (PCR), microarrays, and in situ hybridization, have proven invaluable in …
[PDF][PDF] CRISPR/Cas9 系统活细胞成像技术进展(特邀)
康玥， 廖雪瑶， 谭向宇， 郭萍， 田训 - 红外与激光工程, 2022 - researching.cn
CRISPR/Cas9 系统因其高效, 操作简便, 物种适应性广等优势被广泛应用于基因编辑领域,
该系统是由靶向目标DNA 序列的引导RNA (sgRNA) 和具有切割酶活性的Cas9 蛋白组成 …
该系统是由靶向目标DNA 序列的引导RNA (sgRNA) 和具有切割酶活性的Cas9 蛋白组成 …
Advances in live cell imaging technology of CRISPR/Cas9 system (invited)
K Yue, L Xueyao, T Xiangyu, G Ping, T Xun - 红外与激光工程, 2022 - irla.cn
CRISPR/Cas9 system are widely used in gene editing due to its high efficiency, simple
operation and wide species adaptability. The system consists of a guide RNA (sgRNA) that …
operation and wide species adaptability. The system consists of a guide RNA (sgRNA) that …
[PDF][PDF] Put on your para-spectacles: the development of optimized CRISPR-Cas13-based approaches to image RNA dynamics in real time
BJ Davis, MR O'Connell - Molecular cell, 2020 - cell.com
Live-cell RNA imaging is a powerful approach to observe the real-time dynamics of RNA
metabolism. Two recent papers describe an optimized fluorescence-based CRISPR-Cas13 …
metabolism. Two recent papers describe an optimized fluorescence-based CRISPR-Cas13 …
CRISPR/Cas 工具的开发和应用
方凯伦， 杨辉 - 科学通报, 2020 - cqvip.com
CRISPR/Cas 系统是细菌, 古菌抵抗外源DNA 或RNA 入侵的免疫系统, 细菌和古菌通过crRNA
和Cas 蛋白识别靶标DNA 或RNA, 并切割这些外源入侵核酸. 目前, CRISPR/Cas …
和Cas 蛋白识别靶标DNA 或RNA, 并切割这些外源入侵核酸. 目前, CRISPR/Cas …
[HTML][HTML] Protocol for dynamic imaging of RNA in living cells by CRISPR-Cas13 system
This protocol uses endonuclease-dead, programmable RNA-guided RNA-targeting Cas13
RNases (d) Cas13 proteins fused with fluorescent proteins to visualize and track RNA …
RNases (d) Cas13 proteins fused with fluorescent proteins to visualize and track RNA …