[Frontiers in Bioscience 9, 255-261, January 1, 2004] 255 ASSESSMENT OF HIV-1 DNA
COPIES PER CELL BY REAL-TIME POLYMERASE CHAIN Page 1 [Frontiers in Bioscience 9 …

The use of HIV-1 DNA quantitation in cellular reservoirs to predict disease progression and
treatment outcome in infected patients is hampered by the lack of standardization among the …

A new estimation method for quantitation of HIV-1 DNA was established by introducing a pre-
quantitation polymerase chain reaction (PCR) before conventional real-time PCR. Two …

An assay that quantifies the amount of human immunodeficiency virus type 1 (HIV-1) DNA in
peripheral blood mononuclear cells has been developed. PCR amplification of the HIV-1 …

Despite the success of antiretroviral therapy, human immunodeficiency virus type 1 (HIV-1)
cannot be eradicated. Quantifying the latent HIV reservoir in patients under successful …

ABSTRACT HIV-2 infection is characterized by a very low replication rate in most cases and
low progression. This necessitates an approach to patient monitoring that differs from that for …

Objective: To validate a quantitative polymerase chain reaction method developed to
measure HIV-1 DNA in peripheral blood mononuclear cells. Design: The assay was used to …

A multiple competitor PCR (mcPCR) was developed to quantify HIV-1 proviral DNA from
peripheral blood mononuclear cells (PBMC). DNA extracted from a mixture of HIV infected …

Background A more time saving, convenient, reproducible, and scalable method is needed
to assess total HIV-1 DNA levels. Methods Frozen whole blood and peripheral blood …

Effective antiretroviral therapy usually leads to undetectable HIV-1 RNA in the plasma.
However, the virus persists in some cells of infected patients as various DNA forms, both …