细胞永生化培养技术一直是个难点, 条件性重编程技术(conditional reprogramming, CR)
可能解决这个问题. CR 技术通过将铯源γ 射线辐照过的Swiss-3T3-J2 小鼠成纤维细胞和人正常 …

条件重编程(conditional reprogramming, CR) 培养可以从人源组织中扩增原代细胞,
并使得原代细胞能够于体外永久传代培养. CR 培养体系的建立的特征主要在于新鲜消化分离的 …

Long-term primary culture of mammalian cells has been always difficult due to unavoidable
senescence. Conventional methods for generating immortalized cell lines usually require …

Historically, it has been difficult to propagate cells in vitro that are derived directly from
human tumors or healthy tissue. However, in vitro preclinical models are essential tools for …

Cell reprogramming is a progress in which the memory of a mature cell is erased and then
the cell develops novel phenotype and function; ultimately, the fate of the cell changes. Cell …

BACKGROUND: The use of cloned human embryos gives rise to ethical issues and allows
scientists to find alternative ways to reverse differentiation of cells into multi/pluripotent stem …

It has been demonstrated that mature cells could switch their fate by the technologies for
reprogramming, such as somatic cell nuclear transfer and induced pluripotent stem cells …

This new volume, as a part of Springer's Stem Cells in Regenerative Medicine series, is
dedicated to review the current progress on different approaches of in vivo reprogramming …

细胞直接重编程技术是近年来兴起的重要生物技术, 其广泛的应用可为疾病的治疗, 模型的构建,
再生医学的研究提供了一个新的方向. 细胞直接重编程技术不同于诱导性多能干细胞技术 …