[HTML][HTML] Probing the active fraction of soil microbiomes using BONCAT-FACS
The ability to link soil microbial diversity to soil processes requires technologies that
differentiate active microbes from extracellular DNA and dormant cells. Here, we use …
differentiate active microbes from extracellular DNA and dormant cells. Here, we use …
16S rRNA/rRNA gene ratios and cell activity staining reveal consistent patterns of microbial activity in plant-associated soil
At any given time, only a subset of microbial community members are active in their
environment. The others are in a state of dormancy, with strongly reduced metabolic rates. It …
environment. The others are in a state of dormancy, with strongly reduced metabolic rates. It …
The life beneath our feet
JK Jansson, JI Prosser - Nature, 2013 - nature.com
Our planet's soils teem with microorganisms that regulate processes from crop productivity to
carbon sequestration. Molecular analysis contributes hugely to the characterization of …
carbon sequestration. Molecular analysis contributes hugely to the characterization of …
High-throughput metagenomic technologies for complex microbial community analysis: open and closed formats
Understanding the structure, functions, activities and dynamics of microbial communities in
natural environments is one of the grand challenges of 21st century science. To address this …
natural environments is one of the grand challenges of 21st century science. To address this …
A global atlas of the dominant bacteria found in soil
M Delgado-Baquerizo, AM Oliverio, TE Brewer… - Science, 2018 - science.org
The immense diversity of soil bacterial communities has stymied efforts to characterize
individual taxa and document their global distributions. We analyzed soils from 237 …
individual taxa and document their global distributions. We analyzed soils from 237 …
Resolving functional diversity in relation to microbial community structure in soil: exploiting genomics and stable isotope probing
EMH Wellington, A Berry, M Krsek - Current Opinion in Microbiology, 2003 - Elsevier
The microbial ecology of soil still presents a challenge to microbiologists attempting to
establish the ways in which bacteria and fungi actively metabolise substrates, link into food …
establish the ways in which bacteria and fungi actively metabolise substrates, link into food …
Culture-independent molecular techniques for soil microbial ecology
PR Hirsch, TH Mauchline, IM Clark - Soil Biology and Biochemistry, 2010 - Elsevier
The advent of nucleic acid-based molecular methods, in particular the polymerase chain
reaction (PCR), has revolutionised the study of soil microbial ecology, previously …
reaction (PCR), has revolutionised the study of soil microbial ecology, previously …
Detecting macroecological patterns in bacterial communities across independent studies of global soils
The emergence of high-throughput DNA sequencing methods provides unprecedented
opportunities to further unravel bacterial biodiversity and its worldwide role from human …
opportunities to further unravel bacterial biodiversity and its worldwide role from human …
Activity-based cell sorting reveals responses of uncultured archaea and bacteria to substrate amendment
Metagenomic studies have revolutionized our understanding of the metabolic potential of
uncultured microorganisms in various ecosystems. However, many of these genomic …
uncultured microorganisms in various ecosystems. However, many of these genomic …
[HTML][HTML] A critical perspective on interpreting amplicon sequencing data in soil ecological research
Microbial community analysis via marker gene amplicon sequencing has become a routine
method in the field of soil research. In this perspective, we discuss technical challenges and …
method in the field of soil research. In this perspective, we discuss technical challenges and …