[HTML][HTML] CRISPR/dCas9 系统在活细胞成像领域的研究进展
林斯雨， 钟星， 马立新， 乔洁， 刘奕 - 生物工程学报, 2021 - cjb.ijournals.cn
研究不同基因, 染色体以及基因与染色体之间的时空关系在遗传学, 发育生物学和生物医学等
领域具有重要意义. CRISPR/Cas9 基因编辑技术具有优异的靶向性, 已经成为应用最广泛的 …
领域具有重要意义. CRISPR/Cas9 基因编辑技术具有优异的靶向性, 已经成为应用最广泛的 …
Advances of CRISPR/dCas9 system in live cell imaging
S Lin, X Zhong, L Ma, J Qiao, Y Liu - Sheng wu Gong Cheng xue …, 2021 - europepmc.org
The study of distinct genes, chromosomes and the spatio-temporal relationships between
them is of great significance in genetics, developmental biology and biomedicine …
them is of great significance in genetics, developmental biology and biomedicine …
[PDF][PDF] CRISPR/Cas9 系统活细胞成像技术进展(特邀)
康玥， 廖雪瑶， 谭向宇， 郭萍， 田训 - 红外与激光工程, 2022 - researching.cn
CRISPR/Cas9 系统因其高效, 操作简便, 物种适应性广等优势被广泛应用于基因编辑领域,
该系统是由靶向目标DNA 序列的引导RNA (sgRNA) 和具有切割酶活性的Cas9 蛋白组成 …
该系统是由靶向目标DNA 序列的引导RNA (sgRNA) 和具有切割酶活性的Cas9 蛋白组成 …
Advances in live cell imaging technology of CRISPR/Cas9 system (invited)
K Yue, L Xueyao, T Xiangyu, G Ping, T Xun - 红外与激光工程, 2022 - irla.cn
CRISPR/Cas9 system are widely used in gene editing due to its high efficiency, simple
operation and wide species adaptability. The system consists of a guide RNA (sgRNA) that …
operation and wide species adaptability. The system consists of a guide RNA (sgRNA) that …
Painting a specific chromosome with CRISPR/Cas9 for live-cell imaging
Visualization of chromosome shapes and dynamics in a live cell is highly desirable and
necessary in many areas of cell biology. For example, the copy number of a particular …
necessary in many areas of cell biology. For example, the copy number of a particular …
Live cell imaging of genomic loci using dCas9-SunTag system and a bright fluorescent protein
H Ye, Z Rong, Y Lin - Protein & cell, 2017 - academic.oup.com
CRISPR-Cas9 (clustered regularly interspaced short palindromic repeats-CRISPR
associated) systems have been harnessed for kinds of genome manipulation, including …
associated) systems have been harnessed for kinds of genome manipulation, including …
CRISPR-Cas9 基因编辑技术对细胞内源蛋白进行荧光标记的实验操作
吴仲胜， 高誉， 杜勇涛， 党颂， 何康敏 - 遗传, 2023 - chinagene.cn
CRISPR-Cas9 是目前广泛应用的基因编辑技术, 可对目的基因进行高效精准编辑,
快速实现目的基因的敲除或敲入. Cas9 蛋白在sgRNA 引导下对靶序列进行剪切并造成DNA …
快速实现目的基因的敲除或敲入. Cas9 蛋白在sgRNA 引导下对靶序列进行剪切并造成DNA …
[HTML][HTML] The Current Landscape of CRISPR-Cas9-based Technologies for Imaging of Genomic Loci in Fixed and Live Cells
Visualization of the spatiotemporal organization and dynamics of the genome in the nucleus
is essential to elucidate how chromatin structure and nuclear function are intertwined. In the …
is essential to elucidate how chromatin structure and nuclear function are intertwined. In the …
Genomes in focus: development and applications of CRISPR‐Cas9 imaging technologies
SC Knight, R Tjian, JA Doudna - … Chemie International Edition, 2018 - Wiley Online Library
The discovery of the CRISPR‐Cas9 endonuclease has enabled facile genome editing in
living cells and organisms. Catalytically inactive Cas9 (dCas9) retains the ability to bind …
living cells and organisms. Catalytically inactive Cas9 (dCas9) retains the ability to bind …
Live‐cell imaging of genomic loci with Cas9 variants
Background Endonuclease‐deactivated clustered regularly interspaced short palindromic
repeats (CRISPR)‐associated nuclease (dCas9) has been repurposed for live‐cell imaging …
repeats (CRISPR)‐associated nuclease (dCas9) has been repurposed for live‐cell imaging …