[PDF][PDF] 令和元年12⽉ 月(理学) 申請
生物科学専攻 - 2019 - repository.dl.itc.u-tokyo.ac.jp
The RNA-guided DNA endonuclease Cas9 cleaves double-stranded DNA targets bearing a
protospacer adjacent motif (PAM) and complementarity to a single-guide RNA (sgRNA). The …
protospacer adjacent motif (PAM) and complementarity to a single-guide RNA (sgRNA). The …
[PDF][PDF] Crystal structure of Staphylococcus aureus Cas9
The RNA-guided DNA endonuclease Cas9 cleaves double-stranded DNA targets with a
protospacer adjacent motif (PAM) and complementarity to the guide RNA. Recently, we …
protospacer adjacent motif (PAM) and complementarity to the guide RNA. Recently, we …
[PDF][PDF] Structural basis for the altered PAM specificities of engineered CRISPR-Cas9
S Hirano, H Nishimasu, R Ishitani, O Nureki - Molecular cell, 2016 - cell.com
The RNA-guided endonuclease Cas9 cleaves double-stranded DNA targets bearing a PAM
(protospacer adjacent motif) and complementarity to the guide RNA. A recent study showed …
(protospacer adjacent motif) and complementarity to the guide RNA. A recent study showed …
[PDF][PDF] Structural plasticity of PAM recognition by engineered variants of the RNA-guided endonuclease Cas9
C Anders, K Bargsten, M Jinek - Molecular cell, 2016 - cell.com
The RNA-guided endonuclease Cas9 from Streptococcus pyogenes (SpCas9) forms the
core of a powerful genome editing technology. DNA cleavage by SpCas9 is dependent on …
core of a powerful genome editing technology. DNA cleavage by SpCas9 is dependent on …
[HTML][HTML] Crystal structure of Cas9 in complex with guide RNA and target DNA
The CRISPR-associated endonuclease Cas9 can be targeted to specific genomic loci by
single guide RNAs (sgRNAs). Here, we report the crystal structure of Streptococcus …
single guide RNAs (sgRNAs). Here, we report the crystal structure of Streptococcus …
[HTML][HTML] Structural insights into a high fidelity variant of SpCas9
M Guo, K Ren, Y Zhu, Z Tang, Y Wang, B Zhang… - Cell research, 2019 - nature.com
The RNA-guided endonucleases of the CRISPR-Cas9 system, including the most widely
used Cas9 from Streptococcus pyogenes (SpCas9), are becoming a robust genome editing …
used Cas9 from Streptococcus pyogenes (SpCas9), are becoming a robust genome editing …
Engineering a Streptococcus Cas9 Ortholog with an RxQ PAM-Binding Motif for PAM-Free Gene Control in Bacteria
The RNA-guided Cas9 endonucleases have revolutionized gene editing and regulation, but
their targeting scope is limited by the protospacer adjacent motif (PAM) requirement. The …
their targeting scope is limited by the protospacer adjacent motif (PAM) requirement. The …
[HTML][HTML] Molecular basis for the PAM expansion and fidelity enhancement of an evolved Cas9 nuclease
W Chen, H Zhang, Y Zhang, Y Wang, J Gan, Q Ji - PLoS Biology, 2019 - journals.plos.org
Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas systems have
been harnessed as powerful genome editing tools in diverse organisms. However, the off …
been harnessed as powerful genome editing tools in diverse organisms. However, the off …
Structure-guided improvement of Cas9 specificity
E Zlotorynski - Nature Reviews Molecular Cell Biology, 2016 - nature.com
The Cas9 endonuclease is guided to its targets by single guide RNAs (sgRNAs). Looking to
improve the DNA targeting-specificity of Cas9, Slaymaker et al. hypothesized that DNA …
improve the DNA targeting-specificity of Cas9, Slaymaker et al. hypothesized that DNA …
[HTML][HTML] Unraveling the mechanisms of PAMless DNA interrogation by SpRY-Cas9
GN Hibshman, JPK Bravo, MM Hooper… - Nature …, 2024 - nature.com
CRISPR-Cas9 is a powerful tool for genome editing, but the strict requirement for an NGG
protospacer-adjacent motif (PAM) sequence immediately next to the DNA target limits the …
protospacer-adjacent motif (PAM) sequence immediately next to the DNA target limits the …