Objective Soil microorganisms play an important role in the vegetable soil ecosystem.
Through 16S rRNA gene cloning library technology to analysis the composition of bacteria …

Soil microbial diversity provides basic function of a soil ecosystem. In this study, the total
DNA of microorganisms was extracted by an indirect method from a typical garden soil of …

Rice is one of the most important crops in the Ningxia region of China, and rice planting
helps to maintain ecosystem balance and prevent land desertification. Soil microbial …

Soil is an important epicenter of biodiversity. Human activity has threatened such
biodiversity through vegetation shift. As the major type of microbes, bacteria have played key …

Rhizospheric soil is one the largest reservoirs of microbial genetic diversity. Before
conducting a large-scale metagenomic analysis of an environment, such as a rhizospheric …

This paper assesses on the four approaches for studying soil microbial diversity, traditional
cultured-based microbiological method, microbial biomarker, Biolog GN and molecular …

Bacteria, actinomycetes and fungi are the three major groups of soil microbes. Soil microbes
play a critical role in ecological and biodegradation processes in petroleum-contaminated …

Techniques based on amplification of 16S rRNA genes for comparing bacterial communities
are now widely used in microbial ecology, but calibration of these techniques with traditional …

China's western Ordos is a distribution area for the extremely precious remnants of ancient
Asian environments, which in endangered plant species and complex ecosystems …

This paper reports the results of soil microbial community structure obtained using molecular
method. Meanwhile all details of this method including DNA extraction and cloning are …