ITS-1 versus ITS-2 pyrosequencing: a comparison of fungal populations in truffle grounds

A Mello, C Napoli, C Murat, E Morin, G Marceddu… - Mycologia, 2011 - Taylor & Francis
In a recent study pyrosequencing of the ribosomal internal transcribed spacer-1 (ITS-1) has
validated the effectiveness of such technology in the survey of soil fungal diversity. Here we …

PCR primers specific for the genus Tuber reveal the presence of several truffle species in a truffle-ground

E Zampieri, A Mello, P Bonfante… - FEMS microbiology …, 2009 - academic.oup.com
Truffles are hypogeous Ascomycete fungi belonging to the genus Tuber and forming fruiting
bodies highly prized for their taste and aroma. The identification of the genus Tuber and its …

Tuber melanosporum, when dominant, affects fungal dynamics in truffle grounds

C Napoli, A Mello, A Borra, A Vizzini, P Sourzat… - New Phytologist, 2010 - JSTOR
The fruiting bodies of the ectomycorrhizal (ECM) fungus Tuber melanosporum are usually
collected in an area devoid of vegetation which is defined as a'burnt area'(brulé in French) …

Soil analysis reveals the presence of an extended mycelial network in a Tuber magnatum truffle-ground

E Zampieri, C Murat, M Cagnasso… - FEMS microbiology …, 2009 - academic.oup.com
Truffles are hypogeous ectomycorrhizal fungi. They belong to the genus Tuber and are
currently considered a hot spot in fungal biology due to their ecological and economic …

Pyrosequencing reveals regional differences in fruitassociated fungal communities

MW Taylor, P Tsai, N Anfang, HA Ross… - Environmental …, 2014 - Wiley Online Library
We know relatively little of the distribution of microbial communities generally. Significant
work has examined a range of bacterial communities, but the distribution of microbial …

Typing truffle species by PCR amplification of the ribosomal DNA spacers

B Henrion, G Chevalier, F Martin - Mycological research, 1994 - Elsevier
Variation within the internal transcribed spacer (ITS) and the intergenic spacer (IGS) of the
ribosomal RNA genes of European species of Tuber was examined by polymerase chain …

Utilizing ITS1 and ITS2 to study environmental fungal diversity using pyrosequencing

C Monard, S Gantner, J Stenlid - FEMS microbiology ecology, 2013 - academic.oup.com
The shorter reads generated by high-throughput sequencing has led to a focus on either the
ITS1 or the ITS2 sublocus in fungal diversity analyses. Our study aimed to determine how …

Truffles: much more than a prized and local fungal delicacy

A Mello, C Murat, P Bonfante - FEMS Microbiology Letters, 2006 - academic.oup.com
Truffles are hypogeous fungi which live in symbiosis with plant host roots in order to
accomplish their life cycle. Some species, such as Tuber magnatum Pico, the 'white truffle' …

Multiplex PCR for the identification of white Tuber species

A Amicucci, C Guidi, A Zambonelli… - FEMS microbiology …, 2000 - academic.oup.com
Species-specific primers selected from the internal transcribed spacer region sequence
were used to set up a multiplex polymerase chain reaction (PCR) able to simultaneously …

A new pair of primers designed for amplification of the ITS region in Tuber species

L Bertini, A Amicucci, D Agostini… - FEMS Microbiology …, 1999 - academic.oup.com
The alignment of the 28S gene of several species of Pezizales allowed to select two pairs of
primers able to amplify the internal transcribed spacer region of ribosomal DNA in …