Water in jet fuels is a big concern for the aeronautical industry, especially at low temperatures, for which ice particles may accrete in the fuel feeding systems, generating high pressure drop and a potential restriction of fuel, causing risk of engine failure. This was eg the case in 2008 with the crash of the Boeing 777-GYMMM, for which two engines failure occurred at the Heathrow London airport. As a result, the Air Accidents Investigation Branch launched several studies in order to better understand and prevent such ice blockage situations. For this purpose, the IFTS has developed its own test loop with a reproducible and repeatable ice particle generating method. It operates from-40 to-5 C at the critical water content in jet fuel for emergency system operations, ie 260 ppm for aeronautical filter performances testing. The main objective of this research work is to characterize the size and shape of the ice particles produced in jet A-1 fuel through the ice clogging method of the IFTS at-20 C (temperature believed to be critical in previous studies). The approach is twofold:(1) 3D image acquisition through X-ray micro-tomography of ice particles to characterize their shape and size distributions.(2) direct dynamic observation of these particles inside a glass-section adapted to the pipes of the test loop by a high-speed camera and correlating these 2D results with those of the 3D method. In this paper, and thanks to the knowledge acquired and combined by the IFTS/CEN/3SR-lab, we present a first-of-a-kind method for ice particles 3D characterization within jet fuels and the obtained results according to the production process parameters (the grinding-time of the centrifugal pump and concentration of the injected water).