The establishment of in vitro naive human pluripotent stem cell cultures opened new
perspectives for the study of early events in human development. The role of several
transcription factors and signaling pathways have been characterized during maintenance
of human naive pluripotency. However, little is known about the role exerted by the
extracellular matrix (ECM) and its three-dimensional (3D) organization. Here, using an
unbiased and integrated approach combining microfluidic cultures with transcriptional …

The establishment of in vitro naïve human pluripotent stem cell cultures opened new
perspectives for the study of early events in human development. The role of several
transcription factors and signaling pathways during maintenance of human naïve
pluripotency has been characterized. However, little is known about the role exerted by the
extracellular matrix and its three-dimensional organization. Here, using an unbiased and
integrated approach combining transcriptional, proteomic and secretome analyses, we …