A Combination of Donor Specific Transfusion and Rapamycin Prolonges Cardiac Allograft Survival in Mice
K Wu, F Xiang, J Yuan, Z Zeng, H Zhou… - Transplantation …, 2008 - Elsevier
BACKGROUND: We sought to investigate the effects of donor-specific transfusion (DST)
combined with rapamycin (RPM) on engraftment and demonstrate the mechanisms.
METHODS: B6 was treated with DST (on day− 8) combined with RPM (from day− 7 to− 1, 1.5
μL/g per day). On day 0, B6 splenocytes were harvested to coculture with Balb/c splenocytes
pretreated with mitomycin C in 1-way mixed lymphocyte cultures (MLC). After 48 hours, the
proliferation, we measured apoptosis and the CD4+ CD25+ T-cell ratio of cultured cells. The …
combined with rapamycin (RPM) on engraftment and demonstrate the mechanisms.
METHODS: B6 was treated with DST (on day− 8) combined with RPM (from day− 7 to− 1, 1.5
μL/g per day). On day 0, B6 splenocytes were harvested to coculture with Balb/c splenocytes
pretreated with mitomycin C in 1-way mixed lymphocyte cultures (MLC). After 48 hours, the
proliferation, we measured apoptosis and the CD4+ CD25+ T-cell ratio of cultured cells. The …
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