Cryptococcus neoformans is an opportunistic fungal pathogen with a defined sexual cycle
for which genetic and molecular techniques are well developed. The entire genome
sequence of one C. neoformans strain is nearing completion. The efficient use of this
sequence is dependent upon the development of methods to perform more rapid genetic
analysis including gene-disruption techniques. A modified PCR overlap technique to
generate targeting constructs for gene disruption that contain large regions of gene …

PCR-based strategy to generate integrative targeting alleles with large regions of homology
| CiNii Research … PCR-based strategy to generate integrative targeting alleles with large
regions of homology …