In the adult organism, cell migration is required for physiological processes such as
angiogenesis and immune surveillance, as well as pathological events such as tumor
metastasis. The adaptor protein and Src substrate Tks5 is necessary for cancer cell
migration through extracellular matrix in vitro and tumorigenicity in vivo. However, a role for
Tks5 during embryonic development, where cell migration is essential, has not been
examined. We used morpholinos to reduce Tks5 expression in zebrafish embryos, and …

… Neural crest derivatives require a Src-Tks5-dependent pathway in vivo. (A) Tg(FoxD3:GFP)
embryos (8 hpf) were treated with either vehicle (DMSO) or SU6656 for 24 hours and
imaged by confocal microscopy to detect neural crest cells. (D= dorsal, V= ventral). Brackets
indicate the position of the somites. … (BC) Embryos at 15 hpf were treated as indicated for
24 hours and analyzed for pigmentation defects. (B) …