… We developed a dual SHOX2:GFP; MYH6:mCherry knockin human embryonic stem cell (…
In sum, our dual reporter system facilitated an effective strategy for deriving human SAN-like …

… The HCN4-EGFP/SHOX2-mCherry dual reporter hiPSC system developed in this study …
GFP, SHOX2, and mCherry mRNA expression, were correlated with each other. HCN4 and GFP …

… activation of a transgenic Bmp4 allele in Shox2 −/− background attenuated DMP … the
Shox2-BMP genetic cascade in the regulation of DMP development. Our results reveal that Shox2 …

… We then created a dual knock-in SHOX2:GFP;MYH6:mCherry hESC reporter line to establish
a highly efficient strategy to derive functional human SAN-like pacemaker cells, which was …

… Together, these results showed that dual positive markers (Shox2 and Hcn4) could define
… , about 46.6% SHOX2:GFP + cells were produced, though only SHOX2 is the cardiac PC-…

… of Shox2 INs, we biocytin-filled identified GFP labeled neurons in Shox2cre; Z/EG spinal
cords. The dendritic trees of Shox2 … None of 28 biocytin-filled Shox2 INs gave rise to axons that …

… We developed an efficient strategy, using a dual SHOX2:GFP; MYH6:mCherry knock-in
reporter line, to generate and purify human pluripotent stem cell-derived SAN cells (hPSC-SAN), …

… We developed an efficient strategy, using a dual SHOX2:GFP; MYH6:mCherry knock-in
reporter line, to generate and purify human pluripotent stem cell-derived SAN cells (hPSC-SAN), …

… ng shox2 MO, sufficient to induce the known bradycardia phenotype [24] and then monitored
visible morphological changes and heart rate of GFP … a dual luciferase assay kit (Promega). …

… control of each of the Gli3 or Shox2 enhancers (mm1179–GFP, hs1586–mCherry, hs741–…
In Shox2 double enhancer reporter embryos, a major accumulation of cells with dual Shox2 …