A computational tool for mitochondrial systems biology has been developed as a simulation
model of E-Cell2, a publicly available simulation system. The general model consists of 58
enzymatic reactions and 117 metabolites, representing the respiratory chain, the TCA cycle,
the fatty acid β-oxidation and the inner-membrane transport system. It is based on previously
published enzyme kinetics studies in the literature; we have successfully integrated and
packaged them into a single large model. The model can be easily extended and modified …

Table 10: Kinetic parameters and their sources (AAC) Parameter class notice kf0 0.9 class 0
velocity model, at mp= 0, kf0= kr0 kr0 0.9 class 0 velocity model, at mp= 0 normalize 2.21
class 0 normalizing factor of kf0, kr0 Kd1 5.9 E-4 class 3 Kd1→ Kd, velocity model, Kd1= Kd2
Kd2 5.9 E-4 class 3 Kd2→ Kd Kd accepts no effects from membrane potential Cf 3.30 class 0
kf0× exp (Cf×∆ Ψ)= kf (∆ Ψ) Cr-3.34 class 0 kr0× exp (Cr×∆ Ψ)= kr (∆ Ψ) T 310.0- absolute
temperature kinetic mechanism see [Krämer and Klingenberg, 1982] rate equation See 7.1 …