The formation of new bone during the process of bone remodeling occurs almost exclusively
at sites of prior bone resorption. In an attempt to discover what regulatory pathways are
utilized by osteoblasts to effect this site-specific formation event we probed components of
an active bone resorption surface with an osteoblast phage expression library. In these
experiments primary cultures of rat osteoblasts were used to construct a phage display
library in T7 phage. Tartrate-resistant acid phosphatase (type V)(TRAP) was used as the bait …