A rapid assay for detection of Rose rosette virus using reverse transcription-recombinase polymerase amplification using multiple gene targets

B Babu, BK Washburn, SH Miller, K Poduch… - Journal of virological …, 2017 - Elsevier
Rose rosette disease caused by Rose rosette virus (RRV; genus Emaravirus) is the most
economically relevant disease of Knock Out® series roses in the US As there are no
effective chemical control options for the disease, the most critical disease management
strategies include the use of virus free clean plants for propagation and early detection and
destruction of infected plants. The current diagnostic techniques for RRV including end-point
reverse transcription-polymerase chain reaction (RT-PCR) and real-time PCR (RT-qPCR) …
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