Co-cultural differences in assertiveness within the United States have not been explored, despite noted regional differences in communication patterns (Andersen, Lustig, & Andersen, 1987). This study examines assertiveness behaviors, focusing on university students from the Upper Midwest (n = 148) and the New York Metropolitan region (n = 159) who completed the Rathus Assertiveness Schedule (Rathus, ). New York Metropolitan respondents reported significantly higher levels of assertive communication than did respondents from the Upper Midwest. Males in the Upper Midwest region reported significantly higher levels of assertive communication than did females in the same region. Implications for regional differences in assertiveness are discussed.