A set of recombinant antigens from Echinococcus granulosus with potential for use in the immunodiagnosis of human cystic hydatid disease
VG Virginio, A Hernandez, MB Rott… - Clinical & …, 2003 - academic.oup.com
Clinical & Experimental Immunology, 2003•academic.oup.com
Several recombinant clones expressing antigens from Echinococcus granulosus were
isolated previously from a parasite cDNA library using cystic hydatid disease (CHD) patients'
sera or rabbit hyperimmune antiserum against a lipoproteic fraction from bovine cyst fluid.
Six of these antigens were expressed in Escherichia coli and the purified recombinant
proteins were tested in enzyme-linked immunosorbent assay (ELISA) for specific IgG with a
panel of sera from patients with surgically confirmed (n= 58) or immunologically diagnosed …
isolated previously from a parasite cDNA library using cystic hydatid disease (CHD) patients'
sera or rabbit hyperimmune antiserum against a lipoproteic fraction from bovine cyst fluid.
Six of these antigens were expressed in Escherichia coli and the purified recombinant
proteins were tested in enzyme-linked immunosorbent assay (ELISA) for specific IgG with a
panel of sera from patients with surgically confirmed (n= 58) or immunologically diagnosed …
Summary
Several recombinant clones expressing antigens from Echinococcus granulosus were isolated previously from a parasite cDNA library using cystic hydatid disease (CHD) patients’ sera or rabbit hyperimmune antiserum against a lipoproteic fraction from bovine cyst fluid. Six of these antigens were expressed in Escherichia coli and the purified recombinant proteins were tested in enzyme-linked immunosorbent assay (ELISA) for specific IgG with a panel of sera from patients with surgically confirmed (n = 58) or immunologically diagnosed (n = 71) CHD. Sera from clinically normal individuals (n = 203) and sera from individuals with other helminthic infections (n = 65) were assayed for the assessment of specificity. A cut-off value was determined by receiver-operating-characteristic plots for each antigen. A recombinant antigen B subunit (AgB8/2) presented the highest sensitivity (93·1%), considering the group of sera from patients with CHD surgically confirmed, and specificity (99·5%) and is proposed as the basis for an immunodiagnostic test. The other recombinant antigens tested presented sensitivities between 58·6% and 89·7%, and three of them were considered of complementary value. In subclass-specific ELISA, different IgG isotypes showed dominance in the response for each of the recombinant antigens. There was a clear predominance of IgG4 response for all antigens tested, indicating that this would be the subclass of choice to be assessed for these recombinant proteins.
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