Accurate digital polymerase chain reaction quantification of challenging samples applying inhibitor-tolerant DNA polymerases
M Sidstedt, EL Romsos, R Hedell, R Ansell… - Analytical …, 2017 - ACS Publications
Digital PCR (dPCR) enables absolute quantification of nucleic acids by partitioning of the
sample into hundreds or thousands of minute reactions. By assuming a Poisson distribution
for the number of DNA fragments present in each chamber, the DNA concentration is
determined without the need for a standard curve. However, when analyzing nucleic acids
from complex matrixes such as soil and blood, the dPCR quantification can be biased due to
the presence of inhibitory compounds. In this study, we evaluated the impact of varying the …
sample into hundreds or thousands of minute reactions. By assuming a Poisson distribution
for the number of DNA fragments present in each chamber, the DNA concentration is
determined without the need for a standard curve. However, when analyzing nucleic acids
from complex matrixes such as soil and blood, the dPCR quantification can be biased due to
the presence of inhibitory compounds. In this study, we evaluated the impact of varying the …
[引用][C] Accurate Digital Polymerase Chain Reaction Quantification of Challenging Samples Applying Inhibitor-Tolerant DNA Polymerases
S Maja, H Ronny, A Ricky, R Peter, H Johannes - 2017
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