DNA comprises molecular information stored via genetic bases (G, C, T, A) and also
epigenetic bases, principally 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC).
Both genetic and epigenetic information are vital to our understanding of biology and
disease states. Most DNA sequencing approaches address either genetics or epigenetics
and thus capture incomplete information. Methods widely used to detect epigenetic DNA
bases typically fail to capture common C-to-T mutations or distinguish 5mC from 5hmC …

… DNA and provides a digital readout of bases, which we exemplify by simultaneous
sequencing of G, C, T, A, 5mC and 5hmC; 6-Letter sequencing. We demonstrate … which
leads to high accuracy for both genetic and epigenetic base calling. The approach is …