Although paper-based colorimetric sensors utilizing enzymatic reactions are well suited for
real-field diagnosis, their widespread use is hindered by signal blurring at the detection spot
due to the action of capillary forces on the liquid and the corresponding membrane. In this
study, we eliminated signal losses commonly observed during enzyme-mediated
colorimetric sensing and achieved pattern-free quantitative analysis of glucose and uric acid
by mixing enzymes and color-forming reagents with chitosan oligosaccharide lactate (COL) …