Recently, wearable antennas are become popular due to the miniaturization of wireless devices. The main advantage of using wearable antennas is that they are part of clothing and easily transmit and receive signals through clothes. These antennas play a vital role in many applications like navigation, tracking, health monitoring, physical training, RFID, medicine, and military. This paper describes the design and development of microstrip patch antenna with different fabric materials as substrates for ISM band applications. The performance characteristics like gain, directivity, return loss, VSWR, and radiation characteristics are simulated and measured by using CST.